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PDB Id
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Protein Name
Method
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Summary
Structure Feature
Experiment
Ligands & Environment
37US
pdb_000037us
10.2210/pdb37us/pdb
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FASTA
PDB
MMCIF
Binary MMCIF
XML
Structure Factors
Full Validation Report
Validation File (XML)
Validation File (CIF)
FASTA Zipped(.gz)
PDB Zipped(.gz)
MMCIF Zipped(.gz)
Binary MMCIF Zipped(.gz)
Structure Factors Zipped(.gz)
Validation File Zipped (.xml.gz)
Validation File Zipped (.cif.gz)
ELECTRON MICROSCOPY
Sample
Fibroblast activation protein alpha in complex with H4 VNAR
Specimen Preperation
Sample Aggregation State
PARTICLE
Vitrification Instrument
FEI VITROBOT MARK IV
Cryogen Name
ETHANE
Sample Vitrification Details
?
3D Reconstruction
Reconstruction Method
SINGLE PARTICLE
Number of Particles
332300
Reported Resolution (Å)
3.01
Resolution Method
FSC 0.143 CUT-OFF
Other Details
?
Refinement Type
Symmetry Type
POINT
Map-Model Fitting and Refinement
ID
1
Refinement Space
REAL
Refinement Protocol
AB INITIO MODEL
Refinement Target
?
Overall B Value
?
Fitting Procedure
?
Details
Ab initial ModelAngelo-generated atomic model was docked into the cryo-EM density and iteratively rebuilt in Coot. Automated real-space refinement was performed in Phenix using stereochemical and noncrystallographic symmetry restraints.
Data Acquisition
Detector Type
GATAN K3 (6k x 4k)
Electron Dose (electrons/Å
2
)
80
Imaging Experiment
Date of Experiment
?
Temprature (Kelvin)
Microscope Model
TFS KRIOS
Minimum Defocus (nm)
500
Maximum Defocus (nm)
2000
Minimum Tilt Angle (degrees)
?
Maximum Tilt Angle (degrees)
?
Nominal CS
2.7
Imaging Mode
BRIGHT FIELD
Specimen Holder Model
FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification
105000
Calibrated Magnification
?
Source
FIELD EMISSION GUN
Acceleration Voltage (kV)
300
Imaging Details
?
Imaging Experiment
Task
Software Package
Version
PARTICLE SELECTION
cisTEM
?
IMAGE ACQUISITION
SerialEM
?
CTF CORRECTION
cisTEM
?
MODEL FITTING
ModelAngelo
?
MODEL FITTING
UCSF ChimeraX
?
INITIAL EULER ASSIGNMENT
cisTEM
?
FINAL EULER ASSIGNMENT
cisTEM
?
CLASSIFICATION
cisTEM
?
RECONSTRUCTION
cisTEM
?
MODEL REFINEMENT
PHENIX
?
MODEL REFINEMENT
Coot
?
Image Processing
CTF Correction Type
CTF Correction Details
Number of Particles Selected
Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION
CTF parameters were estimated in cisTEM and subsequently refined using per-particle defocus and beam-tilt refinement. CTF correction was applied during Fourier-space reconstruction.
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