ELECTRON MICROSCOPY

Sample

Bacillus subtilis GerAA(A318C):GerAB:GerAC(S56C) bound to D-alanine, in glyco-diosgenin

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details wait time = 10 s blot time = 6 s blot force above calibrated 0 = +23
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 325035
Reported Resolution (Å) 2.00
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 50.3
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 700
Maximum Defocus (nm) 2200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 5.0.6
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 5.0.6
MODEL REFINEMENT PHENIX 2.1
MODEL REFINEMENT Coot 1.3.1
INITIAL EULER ASSIGNMENT cryoSPARC 5.0.6
FINAL EULER ASSIGNMENT cryoSPARC 5.0.6
RECONSTRUCTION cryoSPARC 5.0.6
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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