ELECTRON MICROSCOPY

Sample

Importin 7 in complex with RanGTP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Sample volume: 2 microliters blotting time: 5 s blot force setting: 6
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 675579
Reported Resolution (Å) 3.60
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 49.2
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 8100
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Warp 1.0.9
CTF CORRECTION Warp 1.0.9
MODEL FITTING UCSF Chimera ?
INITIAL EULER ASSIGNMENT RELION 5.0.1
FINAL EULER ASSIGNMENT RELION 5.0.1
CLASSIFICATION RELION 5.0.1
RECONSTRUCTION RELION 5.0.1
MODEL REFINEMENT PHENIX 2.0_5885
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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