SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 2D 1H-15N HSQC 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
2 2D 1H-13C HSQC 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
3 2D 1H-1H TOCSY 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
4 2D 1H-1H NOESY 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
5 3D 1H-15N NOESY 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
6 3D 1H-13C NOESY 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O 0.1 90% H2O/10% D2O 5-7 ambient 288
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
NMR Refinement
Method Details Software
simulated annealing ? 1
NMR Ensemble Information
Conformer Selection Criteria structures with the least restraint violations
Conformers Calculated Total Number 400
Conformers Submitted Total Number 20
Representative Model 1 (fewest violations)
Computation: NMR Software
# Classification Version Software Name Author
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