ELECTRON MICROSCOPY

Sample

St. Louis encephalitis virus

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument ?
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 49349
Reported Resolution (Å) 3.30
Resolution Method FSC 0.143 CUT-OFF
Other Details The 49,349 viral particles were further processed through localized reconstruction, yielding a subset of 1,172,175 subparticles for the final reconstruction
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON III (4k x 4k)
Electron Dose (electrons/Å2) 30
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1500
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 4
IMAGE ACQUISITION EPU ?
CTF CORRECTION CTFFIND ?
MODEL FITTING UCSF Chimera 1.19
INITIAL EULER ASSIGNMENT RELION 4
FINAL EULER ASSIGNMENT RELION 4
CLASSIFICATION RELION 4
RECONSTRUCTION RELION 4
MODEL REFINEMENT PHENIX 2.0_5885
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING ONLY ?
Feedback Form
Name
Email
Institute
Feedback