ELECTRON MICROSCOPY

Sample

Head-On RNAP-RNAP Collision Elongation Complex: 26 base pairs inter-RNAP distance.

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 219123
Reported Resolution (Å) 2.65
Resolution Method FSC 0.143 CUT-OFF
Other Details Particles were symmetry expanded and then refined with a mask representative of one of the two complexes.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 48.79
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7.0
IMAGE ACQUISITION SerialEM ?
CTF CORRECTION cryoSPARC 4.7.0
MODEL FITTING ISOLDE 1.10.0
MODEL FITTING Coot 0.8.7
MODEL REFINEMENT PHENIX "2.0_5936"
INITIAL EULER ASSIGNMENT cryoSPARC 4.7.0
FINAL EULER ASSIGNMENT cryoSPARC 4.7.0
CLASSIFICATION cryoSPARC 4.7.0
RECONSTRUCTION cryoSPARC 4.7.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
Feedback Form
Name
Email
Institute
Feedback