ELECTRON MICROSCOPY

Sample

Human airway ciliary transition zone (TZ) linker

Specimen Preperation
Sample Aggregation State CELL
Vitrification Instrument ?
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SUBTOMOGRAM AVERAGING
Number of Particles 12237
Reported Resolution (Å) 9.6
Resolution Method FSC 0.143 CUT-OFF
Other Details The deposited volume is a composite map. The general TZ linker was refined to 9.6 using 12,237 subtomograms. The main part of TZ linker was refined to 8.1 using 6,373 subtomograms. The region of TZ linker interacting with A tubule was refined to 6.9 using 10,395 subtomograms. The region of TZ linker interacting with B tubule was refined to 7.0 using 12,780 subtomograms.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 4.68
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 2000
Maximum Defocus (nm) 4000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
VOLUME SELECTION Warp 2
MODEL REFINEMENT PHENIX 2.0_5936
RECONSTRUCTION RELION 5
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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