ELECTRON MICROSCOPY

Sample

This is a complex of domain-swapped dimer of pSTAT1:importin aplha 5 heterodimers.

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 146431
Reported Resolution (Å) 3.6
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target Cross correlation
Overall B Value ?
Fitting Procedure ?
Details Initial fitting in Chimera, followed by rebuilding in Coot and real-space refinement in Phenix.
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz 0.3.0
CTF CORRECTION cryoSPARC v5.0.7
MODEL FITTING UCSF ChimeraX 1.11.1
MODEL REFINEMENT PHENIX 2.0_5936
INITIAL EULER ASSIGNMENT cryoSPARC v5.0.7
FINAL EULER ASSIGNMENT cryoSPARC v5.0.7
CLASSIFICATION cryoSPARC v5.0.7
RECONSTRUCTION cryoSPARC v4.2.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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