ELECTRON MICROSCOPY

Sample

Compound 4 induced DDB1dB/CRBN, KAT2A-NTD ternary complex

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details Grids were vitrified using a Leica EM GP plunge freezer operated at 90% humidity and 10C. Grids were first pre-incubated with 4uL of 10uM CRBN-agnostic IKZF1_140-196_Q146A,G151N for 1 minute and then back-blotted for 4s. Afterwards, 4uL of mixture was applied to the grids, followed by blotting for 4s and plunging into liquid ethane at -181 C.
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 613163
Reported Resolution (Å) 2.90
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 55.8
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details The grid was imaged at a 30-degree tilt
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz ?
MODEL REFINEMENT PHENIX 1.21.2_5419
CTF CORRECTION cryoSPARC ?
INITIAL EULER ASSIGNMENT cryoSPARC ?
FINAL EULER ASSIGNMENT cryoSPARC ?
RECONSTRUCTION cryoSPARC ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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