9ZIP image
Deposition Date 2025-12-04
Release Date 2026-07-22
Last Version Date 2026-07-22
Entry Detail
PDB ID:
9ZIP
Keywords:
HYDROLASE
Title:
Crystal Structure of RASProtease(II), a Designed RAS-specific Subtilisin, in Complex with the Cognate Peptide QEEYSAM
Biological Source:
Source Organism(s):
Bacillus amyloliquefaciens (Taxon ID: 1390)
synthetic construct (Taxon ID: 32630)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
1.24 Å
R-Value Free:
0.13
R-Value Work:
0.11
R-Value Observed:
0.11
Space Group:
P 1 21 1
9ZIP validation report missing
Macromolecular Entities
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Peptide QEEYSAM
Chain IDs:C (auth: B), D (auth: C)
Chain Length:7
Number of Molecules:2
Biological Source:synthetic construct
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:SUBTILISIN BPN'
Chain IDs:A (auth: S), B (auth: A)
Chain Length:268
Number of Molecules:2
Biological Source:Bacillus amyloliquefaciens
Ligand Molecules
ACT
EDO
GOL
K
Primary Citation
Substrate specificity in a designed RAS-targeting protease is coupled to active site and distal motions.
Biorxiv ? ? ? (2026)
PMID: 41648245 DOI: 10.64898/2026.01.15.699477
Primary Citation of related structures
9ZIP
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