ISDA: 9ZIO

Deposition Date 2025-12-04

Release Date 2026-07-22

Last Version Date 2026-07-22

Entry Detail

PDB ID:

9ZIO

Keywords:

HYDROLASE

Title:

Crystal Structure of RASProtease(II), a Designed RAS-specific Subtilisin

Biological Source:

Source Organism(s):

Bacillus amyloliquefaciens (Taxon ID: 1390)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.49 Å

R-Value Free:

0.15

R-Value Work:

0.12

R-Value Observed:

0.12

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:SUBTILISIN BPN'
Chain IDs:A (auth: S), B (auth: A)
Chain Length:268
Number of Molecules:2
Biological Source:Bacillus amyloliquefaciens

Ligand Molecules

EDO

FMT

GOL

PEG

Primary Citation

Substrate specificity in a designed RAS-targeting protease is coupled to active site and distal motions.

Chu, B, He, Y, Chen, Y, Toth, E.A, Orban, J.Show

Biorxiv ? ? ? (2026)

PMID: 41648245 DOI: 10.64898/2026.01.15.699477

Primary Citation of related structures

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