ISDA: 9Z7A

Deposition Date 2025-11-16

Release Date 2026-06-03

Last Version Date 2026-06-03

Entry Detail

PDB ID:

9Z7A

Keywords:

HYDROLASE/IMMUNE SYSTEM

Title:

Cryo-EM structure of Secreted extracellular protein A (SepA) from Shigella flexneri complexed with the fragment antigen binding domain of monoclonal antibody 40

Biological Source:

Source Organism(s):

Shigella flexneri 2a str. 2457T (Taxon ID: 198215)
Homo sapiens (Taxon ID: 9606)

Expression System(s):

Escherichia coli dh5[alpha], Homo sapiens

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

1.99 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Serine protease SepA autotran
Gene (Uniprot):sepA
Mutagens:F684S natural variant compared to Q8VSL2 UniProt entry (F740 in UniProt)
Chain IDs:A
Chain Length:1033
Number of Molecules:1
Biological Source:Shigella flexneri 2a str. 2457T

UniProt ID:Q8VSL2 Pfam ID:PF02395, PF24078 EC:3.4.21.-

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Heavy chain of the fragment a
Chain IDs:B
Chain Length:224
Number of Molecules:1
Biological Source:Homo sapiens

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Light chain of the fragment a
Chain IDs:C
Chain Length:215
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules

Primary Citation

Human infection with enterotoxigenic E. coli elicits antibodies that broadly neutralize mucin-degrading proteases of pathogenic E. coli and Shigella

Buckley, D.P, Akhtar, M, Thapa, M, Schmitz, A, Turner, J, Vickers, T.J, Khatoon, N, Kaisar, H, Coggin, J.A, Ganguli, D, Sheikh, A, Laird, R.M, Poly, F, Porter, C.K, Ruiz-Perez, F, Miller, M.J, Bhuiyan, T.R, Qadri, F, Trillo-Muyo, S, Dolan, B, van der Post, S, Ellebedy, A, Berndsen, Z.T, Fleckenstein, J.M.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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