ISDA: 9YYH

Deposition Date 2025-10-29

Release Date 2026-07-15

Last Version Date 2026-07-15

Entry Detail

PDB ID:

9YYH

Keywords:

RNA BINDING PROTEIN

Title:

AI-generated RNA-guided nuclease R-loop formed state

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)
Deinococcus radiodurans (Taxon ID: 1299)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.80 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:AI-generated RNA-guided nucle
Chain IDs:A
Chain Length:408
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (132-MER)
Chain IDs:B
Chain Length:132
Number of Molecules:1
Biological Source:Deinococcus radiodurans

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*CP*TP*AP*TP*TP*GP
Chain IDs:C
Chain Length:8
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*GP*CP*CP*AP*AP*GP*
Chain IDs:D
Chain Length:20
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*CP*CP*CP*C)-3')
Chain IDs:E
Chain Length:4
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structure and evolution-guided design of minimal RNA-guided nucleases

Skopintsev, P, Esain-Garcia, I, Doudna, J.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

Feedback Form