ISDA: 9YXV

Deposition Date 2025-10-27

Release Date 2026-05-27

Last Version Date 2026-05-27

Entry Detail

PDB ID:

9YXV

Keywords:

IMMUNE SYSTEM

Title:

Cryo-EM structure of the core region of cIL-U1A-Fab1R-PGA1-sfFab quaternary complex at 2.9 A resolution

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)
Streptococcus sp. 'group G' (Taxon ID: 1320)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.90 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:U1 small nuclear ribonucleopr
Gene (Uniprot):SNRPA
Mutagens:Y31H, Q36R
Chain IDs:A
Chain Length:97
Number of Molecules:1
Biological Source:Homo sapiens

UniProt ID:P09012 Pfam ID:PF00076

Polymer Type:polyribonucleotide
Molecule:Hairpin II of the U1 snRNA (U
Chain IDs:B
Chain Length:18
Number of Molecules:1
Biological Source:synthetic construct

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Protein G
Chain IDs:C
Chain Length:67
Number of Molecules:1
Biological Source:Streptococcus sp. 'group G'

Protein Blast

Polymer Type:polypeptide(L)
Molecule:sfFab18 light chain
Chain IDs:D
Chain Length:239
Number of Molecules:1
Biological Source:Homo sapiens

Protein Blast

Polymer Type:polypeptide(L)
Molecule:sfFab18 heavy chain
Chain IDs:E
Chain Length:234
Number of Molecules:1
Biological Source:Homo sapiens

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Fab1R heavy chain
Chain IDs:F (auth: H)
Chain Length:264
Number of Molecules:1
Biological Source:Homo sapiens

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Fab1R light chain
Chain IDs:G (auth: L)
Chain Length:219
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules

DMU

Primary Citation

A universal Fab targeting a conserved U1A-RNA epitope for RNA structure determination by cryo-EM

Filippova, E.V, Krochmal, D, Mukherjee, S, Piccirilli, J.A, Kossiakoff, A.A.Show

Nucleic Acids Res. 54 ? ? (2026)

DOI: 10.1093/nar/gkag502

Primary Citation of related structures

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