ISDA: 9YMY

Deposition Date 2025-10-10

Release Date 2026-04-29

Last Version Date 2026-04-29

Entry Detail

PDB ID:

9YMY

Keywords:

TRANSCRIPTION

Title:

De novo initial transcribing RNA polymerase with 9-mer RNA (RPitc9)

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)
Escherichia phage T7 (Taxon ID: 10760)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.10 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:F (auth: G), G (auth: H)
Chain Length:329
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:P0A7Z4 Pfam ID:PF01193, PF01000, PF03118 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:E (auth: I)
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8V2 Pfam ID:PF04563, PF04561, PF04565, PF10385, PF00562, PF04560 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:H (auth: J)
Chain Length:1415
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8T7 Pfam ID:PF04997, PF00623, PF04983, PF05000, PF04998 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:A (auth: K)
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A800 Pfam ID:PF01192 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA polymerase sigma factor R
Gene (Uniprot):rpoD
Chain IDs:I (auth: L)
Chain Length:616
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P00579 Pfam ID:PF03979, PF00140, PF04546, PF04542, PF04539, PF04545

Polymer Type:polydeoxyribonucleotide
Molecule:T7A1 promoter fragment non-te
Chain IDs:B (auth: P)
Chain Length:97
Number of Molecules:1
Biological Source:Escherichia phage T7

Polymer Type:polydeoxyribonucleotide
Molecule:T7A1 promoter fragment templa
Chain IDs:C (auth: Q)
Chain Length:97
Number of Molecules:1
Biological Source:Escherichia phage T7

Polymer Type:polyribonucleotide
Molecule:Nascent RNA
Chain IDs:D (auth: R)
Chain Length:9
Number of Molecules:1
Biological Source:Escherichia coli

Ligand Molecules

Primary Citation

Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity

Mueller, A.U, Darst, S.A.Show

Mol.Cell 86 ? ? (2026)

DOI: 10.1016/j.molcel.2026.03.033

Primary Citation of related structures

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