ISDA: 9YKW

Deposition Date 2025-10-07

Release Date 2026-08-12

Last Version Date 2026-08-12

Entry Detail

PDB ID:

9YKW

Keywords:

TRANSFERASE/Ligase

Title:

Cryo-EM structure of double-loaded human UBA6-UBE2Z-Ub(t)/Ub(a) thioester mimetic complex.

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)

Expression System(s):

Trichoplusia ni, Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.86 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Ubiquitin-like modifier-activ
Chain IDs:A
Chain Length:1016
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Ubiquitin
Chain IDs:B, D
Chain Length:76
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Ubiquitin-conjugating enzyme
Mutagens:C100S, Y182K, K186R, C261S, C263S
Chain IDs:C
Chain Length:262
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules

IHP

Primary Citation

Cryo-EM structures of UBA6 reveal mechanisms of E1-E2 specificity and dual FAT10/ubiquitin thioester transfer.

Nayak, D, Jia, L, Dos Santos Bury, P, Ruben, E.A, Shukla, A, Nayak, A, Stratton, C.M, Ebadi, P, Cho, H, Tumanova, A.A, Varughese, J.T, Yuan, L, Gao, F, Cano, K.E, Davies, C, Sung, P, Gack, M.U, Wasmuth, E.V, Olsen, S.K.Show

Nat Commun 17 ? ? (2026)

PMID: 41764162 DOI: 10.1038/s41467-026-69882-3

Primary Citation of related structures

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