ISDA: 9WNE

Deposition Date 2025-09-04

Release Date 2026-10-14

Last Version Date 2026-10-14

Entry Detail

PDB ID:

9WNE

Keywords:

RNA BINDING PROTEIN

Title:

Cryo-EM structure of the LbCas12a-Lid2-crRNA-DNA complex

Biological Source:

Source Organism(s):

Lachnospiraceae bacterium ND2006 (Taxon ID: 1410628)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.54 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cpf1
Chain IDs:A
Chain Length:1237
Number of Molecules:1
Biological Source:Lachnospiraceae bacterium ND2006

UniProt ID:A0A182DWE3

Polymer Type:polyribonucleotide
Molecule:crRNA
Chain IDs:B
Chain Length:42
Number of Molecules:1
Biological Source:Lachnospiraceae bacterium ND2006

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:C
Chain Length:39
Number of Molecules:1
Biological Source:Lachnospiraceae bacterium ND2006

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:D
Chain Length:39
Number of Molecules:1
Biological Source:Lachnospiraceae bacterium ND2006

Ligand Molecules

Primary Citation

Engineering and characterization of a novel Cas12a nickase

Warren Villegas, R, Omura, S.N, Castro, D.R, Sudfeld, C, Luteijn, M, Morinaga, H, Inoue, W.J, Shuto, Y, Vleesschauwer, D.D, Staals, R.H.J, Nureki, O, Wu, W.Y, Van der Oost, J.Show

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Primary Citation of related structures

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