9WHX image
Deposition Date 2025-08-27
Release Date 2026-08-12
Last Version Date 2026-08-26
Entry Detail
PDB ID:
9WHX
Title:
Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, left-half DNA, and right-half DNA in the pre-strand exchange state
Biological Source:
Source Organism(s):
Escherichia coli (Taxon ID: 562)
Expression System(s):
Method Details:
Experimental Method:
Resolution:
2.50 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
Macromolecular Entities
Polymer Type:polypeptide(L)
Molecule:IS621 transposase
Chain IDs:A, B, C, D
Chain Length:328
Number of Molecules:4
Biological Source:Escherichia coli
Polymer Type:polyribonucleotide
Molecule:bridge RNA
Chain IDs:E, F
Chain Length:180
Number of Molecules:2
Biological Source:Escherichia coli
Polymer Type:polydeoxyribonucleotide
Molecule:LH, top strand
Chain IDs:G
Chain Length:38
Number of Molecules:1
Biological Source:Escherichia coli
Polymer Type:polydeoxyribonucleotide
Molecule:LH, bottom strand
Chain IDs:H
Chain Length:38
Number of Molecules:1
Biological Source:Escherichia coli
Polymer Type:polydeoxyribonucleotide
Molecule:RH, top strand
Chain IDs:I
Chain Length:44
Number of Molecules:1
Biological Source:Escherichia coli
Polymer Type:polydeoxyribonucleotide
Molecule:RH, bottom strand
Chain IDs:J
Chain Length:44
Number of Molecules:1
Biological Source:Escherichia coli
Ligand Molecules
Primary Citation
Structural mechanism governing the directionality of bridge recombination.
Nature ? ? ? (2026)
PMID: 42587149 DOI: 10.1038/s41586-026-10903-y
Primary Citation of related structures
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