ISDA: 9W6T

Deposition Date 2025-08-05

Release Date 2026-08-12

Last Version Date 2026-08-12

Entry Detail

PDB ID:

9W6T

Keywords:

GENE REGULATION/DNA

Title:

Cryo-EM structure of a stacked human nucleosome core particle dimer (type 5) assembled with DNA truncated at SHL-5.5

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)
Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

7.61 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Histone H3.1
Chain IDs:A, E, K, O
Chain Length:96
Number of Molecules:4
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Histone H4
Chain IDs:B, F, L, P
Chain Length:84
Number of Molecules:4
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Histone H2A type 1-D
Chain IDs:C, G, M, Q
Chain Length:102
Number of Molecules:4
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Histone H2B type 1-B
Chain IDs:D, H, N
Chain Length:87
Number of Molecules:3
Biological Source:Homo sapiens

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (128-MER)
Chain IDs:I, S
Chain Length:128
Number of Molecules:2
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (128-MER)
Chain IDs:J, T
Chain Length:128
Number of Molecules:2
Biological Source:Escherichia coli

Polymer Type:polypeptide(L)
Molecule:Histone H2B type 1-J
Chain IDs:R
Chain Length:85
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules

Primary Citation

Structural basis for nucleosome stacking diversity mediated by solvent-exposed histone interfaces

Mu, Z, Huang, J.Show

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