ISDA: 9W3E

Deposition Date 2025-07-29

Release Date 2026-04-29

Last Version Date 2026-04-29

Entry Detail

PDB ID:

9W3E

Keywords:

TRANSCRIPTION

Title:

Cryo-EM structure of E. coli RNA polymerase in complex with PP1

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)
Pseudomonas phage vB_PaeM_PAO1_Ab17 (Taxon ID: 1548904)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.60 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:A, B
Chain Length:329
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:C3SR67

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:C
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:C3SIA7

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:D
Chain Length:1407
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:C3SIA2

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:F (auth: E)
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:C3SM87

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Phage protein
Chain IDs:E (auth: G)
Chain Length:124
Number of Molecules:1
Biological Source:Pseudomonas phage vB_PaeM_PAO1_Ab17

UniProt ID:9W3E

Ligand Molecules

Primary Citation

AlphaFold 3-powered discovery of phage proteins that inhibit bacterial transcription initiation.

Yuan, L, Liu, Q, Xiao, X, Xu, L, Liang, L, Guo, Y, Yao, Y, Wang, H, Feng, Y, Hua, X, Feng, Y.Show

Cell Rep 45 117082 117082 (2026)

PMID: 41824451 DOI: 10.1016/j.celrep.2026.117082

Primary Citation of related structures

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