ISDA: 9VW8

Deposition Date 2025-07-16

Release Date 2026-07-22

Last Version Date 2026-07-22

Entry Detail

PDB ID:

9VW8

Keywords:

GENE REGULATION/DNA

Title:

Cryo-EM structure of E.coli transcription initiation complex with Escherichia phage Mu late transcription activator C using the PI promoter DNA

Biological Source:

Source Organism(s):

Escherichia coli K-12 (Taxon ID: 83333)
Escherichia phage Mu (Taxon ID: 2681603)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.36 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (73-MER)
Chain IDs:A (auth: 1)
Chain Length:73
Number of Molecules:1
Biological Source:Escherichia coli K-12

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (73-MER)
Chain IDs:B (auth: 2)
Chain Length:73
Number of Molecules:1
Biological Source:Escherichia coli K-12

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:C (auth: A), D (auth: B), J (auth: P)
Chain Length:329
Number of Molecules:3
Biological Source:Escherichia coli K-12

UniProt ID:P0A7Z4 Pfam ID:PF01193, PF01000, PF03118 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:E (auth: C)
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli K-12

UniProt ID:P0A8V2 Pfam ID:PF04563, PF04561, PF04565, PF10385, PF00562, PF04560 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:F (auth: D)
Chain Length:1407
Number of Molecules:1
Biological Source:Escherichia coli K-12

UniProt ID:P0A8T7 Pfam ID:PF04997, PF00623, PF04983, PF05000, PF04998 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:G (auth: E)
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli K-12

UniProt ID:P0A800 Pfam ID:PF01192 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA polymerase sigma factor R
Gene (Uniprot):rpoD
Chain IDs:H (auth: F)
Chain Length:613
Number of Molecules:1
Biological Source:Escherichia coli K-12

UniProt ID:P00579 Pfam ID:PF03979, PF00140, PF04546, PF04542, PF04539, PF04545

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Late transcription activator
Gene (Uniprot):C
Chain IDs:I (auth: J), K (auth: I), L (auth: K), M (auth: L)
Chain Length:160
Number of Molecules:4
Biological Source:Escherichia phage Mu

UniProt ID:P06022 Pfam ID:PF08765

Ligand Molecules

Primary Citation

Cryo-EM structure of E.coli transcription initiation complex with Escherichia phage Mu late transcription activator C using the PI promoter DNA

Lin, W.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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