ISDA: 9UD1

Deposition Date 2025-04-06

Release Date 2026-10-14

Last Version Date 2026-10-14

Entry Detail

PDB ID:

9UD1

Keywords:

DNA BINDING PROTEIN

Title:

Cryo-EM structure of STITCHR (R2Tg and Cas9 nickase) bound to guide RNA, template RNA, and target DNA in the target-primed reverse transcription initiation state

Biological Source:

Source Organism(s):

Taeniopygia guttata (Taxon ID: 59729)
Streptococcus pyogenes (Taxon ID: 1314)
Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA (Taxon ID: 1531949)

Expression System(s):

Spodoptera frugiperda, Escherichia coli, In vitro transcription vector pt7-tp(deltai)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.30 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Reverse transcriptase-like pr
Chain IDs:A
Chain Length:1287
Number of Molecules:1
Biological Source:Taeniopygia guttata

Polymer Type:polyribonucleotide
Molecule:R2Tg 3'UTR RNA
Chain IDs:B
Chain Length:78
Number of Molecules:1
Biological Source:Taeniopygia guttata

Polymer Type:polydeoxyribonucleotide
Molecule:DNA non-target strand (bound
Chain IDs:C, H
Chain Length:60
Number of Molecules:2
Biological Source:Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA

Polymer Type:polydeoxyribonucleotide
Molecule:DNA non-target strand (bound
Chain IDs:D, I
Chain Length:20
Number of Molecules:2
Biological Source:Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA

Polymer Type:polydeoxyribonucleotide
Molecule:DNA non target strand (primin
Chain IDs:E
Chain Length:41
Number of Molecules:1
Biological Source:Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):dcas9
Chain IDs:F
Chain Length:1367
Number of Molecules:1
Biological Source:Streptococcus pyogenes

UniProt ID:A0A386IRG9 EC:3.1.-.-

Polymer Type:polyribonucleotide
Molecule:guide RNA
Chain IDs:G
Chain Length:98
Number of Molecules:1
Biological Source:Streptococcus pyogenes

Ligand Molecules