ISDA: 9U9L

Deposition Date 2025-03-28

Release Date 2026-04-01

Last Version Date 2026-07-15

Entry Detail

PDB ID:

9U9L

Keywords:

TRANSFERASE

Title:

Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Trp185Tyr/His280Ala with 2-fluoro-3-phenylpropanoic acid

Biological Source:

Source Organism(s):

Rhodopseudomonas palustris CGA009 (Taxon ID: 258594)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.47 Å

R-Value Free:

0.19

R-Value Work:

0.17

R-Value Observed:

0.17

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Fluoroacetate dehalogenase
Gene (Uniprot):RPA1163
Mutagens:W181Y,H280A
Chain IDs:A, B
Chain Length:298
Number of Molecules:2
Biological Source:Rhodopseudomonas palustris CGA009

UniProt ID:Q6NAM1 EC:3.8.1.3

Modified Residue

Compound ID	Chain ID	Parent Comp ID	Details	2D Image
A1ER5	A	ASP	modified residue

Primary Citation

Creating highly active fluoroacetate dehalogenases via gate-based synergetic chain design.

Wang, C.Z, Huang, H, Hu, Z, Gao, L, Lin, Z.Q, Shi, M, Song, Z, Li, M, Wang, Y, Chen, K.W, Liu, Y, Ma, Y, Chen, B, Yan, Q, Zhang, Z.M, Wang, J.B.Show

Nat Commun 17 ? ? (2026)

PMID: 42062289 DOI: 10.1038/s41467-026-72741-w

Primary Citation of related structures

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