ISDA: 9TQ4

Deposition Date 2025-12-19

Release Date 2026-08-19

Last Version Date 2026-08-19

Entry Detail

PDB ID:

9TQ4

Keywords:

HYDROLASE

Title:

Asp2Cas12l-sgRNA bound to target DNA

Biological Source:

Source Organism(s):

Armatimonadota (Taxon ID: 67819)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.51 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Asp2Cas12l
Chain IDs:A
Chain Length:867
Number of Molecules:1
Biological Source:Armatimonadota

Polymer Type:polyribonucleotide
Molecule:sgRNA (149-MER)
Chain IDs:B
Chain Length:164
Number of Molecules:1
Biological Source:Armatimonadota

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (26-MER)
Chain IDs:C
Chain Length:47
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (34-MER)
Chain IDs:D
Chain Length:47
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

A Potent CRISPR-Cas12l Double-Strand Break Gene Editor.

Urbaitis, T, Trinkuniene, L, Lenkaite, I, Petrauskyte, M, Krasauskas, R, Stitilyte, M, Sabaliauskas, M, Sasnauskas, G, Tamulaitiene, G, Young, J.K, Siksnys, V, Gasiunas, G.Show

CRISPR J 9 126 140 (2026)

PMID: 42163774 DOI: 10.1177/25731599261448428

Primary Citation of related structures

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