9SWN image
Deposition Date 2025-10-07
Release Date 2026-04-08
Last Version Date 2026-04-08
Entry Detail
PDB ID:
9SWN
Keywords:
RNA BINDING PROTEIN
Title:
Cryo-EM structure of yeast telomerase holoenzyme
Biological Source:
Source Organism(s):
Saccharomyces cerevisiae (Taxon ID: 4932)
Expression System(s):
Saccharomyces cerevisiae
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.00 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9SWN validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Telomerase reverse transcript
Gene (Uniprot):EST2
Chain IDs:G (auth: A)
Chain Length:884
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:Q06163 EC:2.7.7.49
Polymer Type:polyribonucleotide
Molecule:TLC1 RNA
Chain IDs:F (auth: B)
Chain Length:1157
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Telomere replication protein
Gene (Uniprot):EST3
Chain IDs:A (auth: C)
Chain Length:181
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:Q03096
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribonucleases P/MRP protein s
Gene (Uniprot):POP1
Chain IDs:E (auth: D)
Chain Length:875
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:P41812 EC:3.1.26.5
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribonucleases P/MRP protein s
Gene (Uniprot):POP6
Chain IDs:B (auth: E)
Chain Length:158
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:P53218 EC:3.1.26.5
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribonucleases P/MRP protein s
Gene (Uniprot):POP7
Chain IDs:D (auth: F)
Chain Length:140
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:P38291 EC:3.1.26.5
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Telomere elongation protein E
Gene (Uniprot):EST1
Chain IDs:C (auth: G)
Chain Length:699
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae
UniProt ID:P17214
Ligand Molecules
MG
ZN
Primary Citation
Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme.
Science 391 eadz5344 eadz5344 (2026)
PMID: 41886584 DOI: 10.1126/science.adz5344

Abstact

Telomerase is a reverse transcriptase that synthesizes telomeric repeats at chromosome ends, safeguarding genome integrity. We present the cryo-electron microscopy structure of the budding yeast telomerase, which exhibits substantial divergence from its ciliate and vertebrate counterparts. The structure reveals a stable core formed by telomerase RNA TLC1; the three ever shorter telomere (Est) proteins, Est1, Est2 and Est3; and the Pop1/Pop6/Pop7 complex (Pop1/6/7). TLC1, Est3, and Pop1/6/7 serve critical roles in complex assembly. We identified a zinc finger (ZnF) motif in the telomerase reverse transcriptase (TERT) subunit Est2 that is crucial for telomerase function. Structure prediction suggests the presence of ZnFs in TERT from diverse species. These findings offer insights into the functional organization of yeast telomerase and underscore the evolutionary diversity of telomerase holoenzymes.

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Primary Citation of related structures
9SWN
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