ISDA: 9Q7Z

Deposition Date 2025-08-25

Release Date 2025-09-10

Last Version Date 2026-07-08

Entry Detail

PDB ID:

9Q7Z

Keywords:

DNA BINDING PROTEIN

Title:

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.32 Å

R-Value Free:

0.28

R-Value Work:

0.23

R-Value Observed:

0.24

Space Group:

C 1 2 1

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:eI-OnuI_P456_CGG
Chain IDs:A, D
Chain Length:300
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (26-MER)
Chain IDs:B, E
Chain Length:26
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (26-MER)
Chain IDs:C, F
Chain Length:26
Number of Molecules:2
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Caveat emptor: predicting and modeling protein-DNA recognition and binding via machine-learning computational approaches.

Esler, M.A, Werther, R, Doyle, L.A, Ubilla-Rodriguez, N.C, Schwensen, J.S, Hallinan, J.P, Lambert, A.R, Young, J.C, Silverstein, M, Stoddard, B.L.Show

Nucleic Acids Res. 54 ? ? (2026)

PMID: 42345194 DOI: 10.1093/nar/gkag608

Primary Citation of related structures

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