ISDA: 9Q6R

Deposition Date 2025-08-22

Release Date 2026-09-09

Last Version Date 2026-09-09

Entry Detail

PDB ID:

9Q6R

Keywords:

RIBOSOME

Title:

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with pleuromutilin-27 triazole derivative and protein Y at 2.85A resolution

Biological Source:

Source Organism(s):

Escherichia coli K-12 (Taxon ID: 83333)
Thermus thermophilus HB8 (Taxon ID: 300852)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.85 Å

R-Value Free:

0.26

R-Value Work:

0.21

R-Value Observed:

0.21

Space Group:

P 21 21 21

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L27
Gene (Uniprot):rpmA
Chain IDs:V (auth: 10), WB (auth: 20)
Chain Length:85
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60493 Pfam ID:PF01016

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L28
Gene (Uniprot):rpmB
Chain IDs:W (auth: 11), XB (auth: 21)
Chain Length:98
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60494 Pfam ID:PF00830

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L29
Gene (Uniprot):rpmC
Chain IDs:X (auth: 12), YB (auth: 22)
Chain Length:72
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP6 Pfam ID:PF00831

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L30
Gene (Uniprot):rpmD
Chain IDs:Y (auth: 13), ZB (auth: 23)
Chain Length:60
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHQ6 Pfam ID:PF00327

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L31
Gene (Uniprot):rpmE
Chain IDs:Z (auth: 14), AC (auth: 24)
Chain Length:71
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SJE1 Pfam ID:PF01197

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L32
Gene (Uniprot):rpmF
Chain IDs:AA (auth: 15), BC (auth: 25)
Chain Length:60
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80339 Pfam ID:PF01783

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L33
Gene (Uniprot):rpmG
Chain IDs:BA (auth: 16), CC (auth: 26)
Chain Length:54
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P35871 Pfam ID:PF00471

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L34
Gene (Uniprot):rpmH
Chain IDs:CA (auth: 17), DC (auth: 27)
Chain Length:49
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80340 Pfam ID:PF00468

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L35
Gene (Uniprot):rpmI
Chain IDs:DA (auth: 18), EC (auth: 28)
Chain Length:65
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SKU1 Pfam ID:PF01632

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L36
Gene (Uniprot):rpmJ
Chain IDs:EA (auth: 19), FC (auth: 29)
Chain Length:37
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHR2 Pfam ID:PF00444

Polymer Type:polyribonucleotide
Molecule:23S Ribosomal RNA
Chain IDs:A (auth: 1A), BB (auth: 2A)
Chain Length:2915
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

Polymer Type:polyribonucleotide
Molecule:5S Ribosomal RNA
Chain IDs:B (auth: 1B), CB (auth: 2B)
Chain Length:121
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L2
Gene (Uniprot):rplB
Chain IDs:C (auth: 1D), DB (auth: 2D)
Chain Length:276
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60405 Pfam ID:PF00181, PF03947

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L3
Gene (Uniprot):rplC
Chain IDs:D (auth: 1E), EB (auth: 2E)
Chain Length:206
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHN8 Pfam ID:PF00297

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L4
Gene (Uniprot):rplD
Chain IDs:E (auth: 1F), FB (auth: 2F)
Chain Length:210
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHN9 Pfam ID:PF00573

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L5
Gene (Uniprot):rplE
Chain IDs:F (auth: 1G), GB (auth: 2G)
Chain Length:182
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHQ0 Pfam ID:PF00281, PF00673

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L6
Gene (Uniprot):rplF
Chain IDs:G (auth: 1H), HB (auth: 2H)
Chain Length:180
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P0DOY8 Pfam ID:PF00347

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L9
Gene (Uniprot):rplI
Chain IDs:H (auth: 1I), IB (auth: 2I)
Chain Length:148
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SLQ1 Pfam ID:PF01281, PF03948

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L13
Gene (Uniprot):rplM
Chain IDs:I (auth: 1N), JB (auth: 2N)
Chain Length:140
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60488 Pfam ID:PF00572

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L14
Gene (Uniprot):rplN
Chain IDs:J (auth: 1O), KB (auth: 2O)
Chain Length:122
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP8 Pfam ID:PF00238

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L15
Gene (Uniprot):rplO
Chain IDs:K (auth: 1P), LB (auth: 2P)
Chain Length:150
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHQ7 Pfam ID:PF00828

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L16
Gene (Uniprot):rplP
Chain IDs:L (auth: 1Q), MB (auth: 2Q)
Chain Length:141
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60489 Pfam ID:PF00252

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L17
Gene (Uniprot):rplQ
Chain IDs:M (auth: 1R), NB (auth: 2R)
Chain Length:118
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q9Z9H5 Pfam ID:PF01196

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L18
Gene (Uniprot):rplR
Chain IDs:N (auth: 1S), OB (auth: 2S)
Chain Length:112
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHQ4 Pfam ID:PF00861

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L19
Gene (Uniprot):rplS
Chain IDs:O (auth: 1T), PB (auth: 2T)
Chain Length:146
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60490 Pfam ID:PF01245

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L20
Gene (Uniprot):rplT
Chain IDs:P (auth: 1U), QB (auth: 2U)
Chain Length:118
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60491 Pfam ID:PF00453

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L21
Gene (Uniprot):rplU
Chain IDs:Q (auth: 1V), RB (auth: 2V)
Chain Length:101
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P60492 Pfam ID:PF00829

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L22
Gene (Uniprot):rplV
Chain IDs:R (auth: 1W), SB (auth: 2W)
Chain Length:113
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP3 Pfam ID:PF00237

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L23
Gene (Uniprot):rplW
Chain IDs:S (auth: 1X), TB (auth: 2X)
Chain Length:96
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP0 Pfam ID:PF00276

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L24
Gene (Uniprot):rplX
Chain IDs:T (auth: 1Y), UB (auth: 2Y)
Chain Length:110
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP9 Pfam ID:PF00467, PF17136

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:50S ribosomal protein L25
Gene (Uniprot):rplY
Chain IDs:U (auth: 1Z), VB (auth: 2Z)
Chain Length:206
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHZ1 Pfam ID:PF01386, PF14693

Polymer Type:polyribonucleotide
Molecule:16S Ribosomal RNA
Chain IDs:FA (auth: 1a), GC (auth: 2a)
Chain Length:1521
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S2
Gene (Uniprot):rpsB
Chain IDs:GA (auth: 1b), HC (auth: 2b)
Chain Length:256
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80371 Pfam ID:PF00318

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S3
Gene (Uniprot):rpsC
Chain IDs:HA (auth: 1c), IC (auth: 2c)
Chain Length:239
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80372 Pfam ID:PF07650, PF00189

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S4
Gene (Uniprot):rpsD
Chain IDs:IA (auth: 1d), JC (auth: 2d)
Chain Length:209
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80373 Pfam ID:PF00163, PF01479

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S5
Gene (Uniprot):rpsE
Chain IDs:JA (auth: 1e), KC (auth: 2e)
Chain Length:162
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHQ5 Pfam ID:PF00333, PF03719

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S6
Gene (Uniprot):rpsF
Chain IDs:KA (auth: 1f), LC (auth: 2f)
Chain Length:101
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SLP8 Pfam ID:PF01250

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S7
Gene (Uniprot):rpsG
Chain IDs:LA (auth: 1g), MC (auth: 2g)
Chain Length:156
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P17291 Pfam ID:PF00177

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S8
Gene (Uniprot):rpsH
Chain IDs:MA (auth: 1h), NC (auth: 2h)
Chain Length:138
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P0DOY9 Pfam ID:PF00410

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S9
Gene (Uniprot):rpsI
Chain IDs:NA (auth: 1i), OC (auth: 2i)
Chain Length:128
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80374 Pfam ID:PF00380

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S10
Gene (Uniprot):rpsJ
Chain IDs:OA (auth: 1j), PC (auth: 2j)
Chain Length:105
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHN7 Pfam ID:PF00338

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S11
Gene (Uniprot):rpsK
Chain IDs:PA (auth: 1k), QC (auth: 2k)
Chain Length:129
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80376 Pfam ID:PF00411

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S12
Gene (Uniprot):rpsL
Chain IDs:QA (auth: 1l), RC (auth: 2l)
Chain Length:132
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHN3 Pfam ID:PF00164

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S13
Gene (Uniprot):rpsM
Chain IDs:RA (auth: 1m), SC (auth: 2m)
Chain Length:126
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80377 Pfam ID:PF00416

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S14 typ
Gene (Uniprot):rpsZ
Chain IDs:SA (auth: 1n), TC (auth: 2n)
Chain Length:61
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P0DOY6 Pfam ID:PF00253

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S15
Gene (Uniprot):rpsO
Chain IDs:TA (auth: 1o), UC (auth: 2o)
Chain Length:89
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SJ76 Pfam ID:PF00312

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S16
Gene (Uniprot):rpsP
Chain IDs:UA (auth: 1p), VC (auth: 2p)
Chain Length:88
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SJH3 Pfam ID:PF00886

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S17
Gene (Uniprot):rpsQ
Chain IDs:VA (auth: 1q), WC (auth: 2q)
Chain Length:105
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P0DOY7 Pfam ID:PF00366

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S18
Gene (Uniprot):rpsR
Chain IDs:WA (auth: 1r), XC (auth: 2r)
Chain Length:88
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SLQ0 Pfam ID:PF01084

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S19
Gene (Uniprot):rpsS
Chain IDs:XA (auth: 1s), YC (auth: 2s)
Chain Length:93
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SHP2 Pfam ID:PF00203

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein S20
Gene (Uniprot):rpsT
Chain IDs:YA (auth: 1t), ZC (auth: 2t)
Chain Length:106
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:P80380 Pfam ID:PF01649

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:30S ribosomal protein Thx
Gene (Uniprot):rpsU
Chain IDs:ZA (auth: 1u), AD (auth: 2u)
Chain Length:27
Number of Molecules:2
Biological Source:Thermus thermophilus HB8

UniProt ID:Q5SIH3 Pfam ID:PF17070

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosome-associated inhibitor
Gene (Uniprot):raiA
Chain IDs:AB (auth: 1y), BD (auth: 2y)
Chain Length:113
Number of Molecules:2
Biological Source:Escherichia coli K-12

UniProt ID:P0AD49 Pfam ID:PF02482

Modified Residue

Compound ID	Chain ID	Parent Comp ID	Details
0TD	QA, RC	ASP	modified residue
2MA	A, BB	A	modified residue
2MG	FA, GC	G	modified residue
4OC	FA, GC	C	modified residue
5MC	A, FA, BB, GC	C	modified residue
5MU	A, BB	U	modified residue
G7M	FA, GC	G	modified residue
M2G	FA, GC	G	modified residue
MA6	FA, GC	A	modified residue
OMC	A, BB	C	modified residue
OMG	A, BB	G	modified residue
OMU	A, BB	U	modified residue
PSU	A, FA, BB, GC	U	modified residue
UR3	FA, GC	U	modified residue