ISDA: 9PVL

Deposition Date 2025-08-01

Release Date 2026-07-15

Last Version Date 2026-09-02

Entry Detail

PDB ID:

9PVL

Keywords:

DE NOVO PROTEIN

Title:

Crystal Structure of Fluorophore-binding Protein JF657-bp

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.44 Å

R-Value Free:

0.24

R-Value Work:

0.21

R-Value Observed:

0.21

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:JF657-bp
Chain IDs:A, B (auth: C), C (auth: D), D (auth: B)
Chain Length:136
Number of Molecules:4
Biological Source:synthetic construct

Ligand Molecules

PE4

PG4

Primary Citation

De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging.

Tran, L, Klein, S, Juergens, D, Sharma, S, Decarreau, J, Lee, G.R, Wang, Y, Chen, W, Bera, A.K, Kang, A, Woods, J, Joyce, E, Vafeados, D.K, Roullier, N, Li, X, Liu, B, Bo, Y, Muratspahic, E, Brown, T.A, Grimm, J.B, Patel, R, Lavis, L.D, Mahamid, J, An, L, Baker, D.Show

Science 393 813 819 (2026)

PMID: 42461986 DOI: 10.1126/science.aeb0822

Primary Citation of related structures

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