ISDA: 9PID

Deposition Date 2025-07-10

Release Date 2026-07-15

Last Version Date 2026-07-15

Entry Detail

PDB ID:

9PID

Keywords:

HYDROLASE

Title:

Crystal structure of an engineered thermostable MHETase, MHT077, W148R variant

Biological Source:

Source Organism(s):

Armatimonadota bacterium (Taxon ID: 2033014)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.24 Å

R-Value Free:

0.21

R-Value Work:

0.17

R-Value Observed:

0.17

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Alpha/beta fold hydrolase
Chain IDs:A, B
Chain Length:252
Number of Molecules:2
Biological Source:Armatimonadota bacterium

UniProt ID:A0A7C2Z7G5

Ligand Molecules

EDO

Primary Citation

Engineering thermostable ferulic acid esterases for MHET hydrolysis

Murphy, N.P, Gado, J.E, Mathews, I.I, Komp, K, Bell, E.L, Norton-Baker, B, Clark, M, Avilan, L, Garcia, R, Alt, H, Sarangi, R, Pickford, A, McGeehan, J.E, Gauthier, N.P, Beckham, G.T.Show

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