ISDA: 9OP9

Deposition Date 2025-05-17

Release Date 2026-05-20

Last Version Date 2026-05-20

Entry Detail

PDB ID:

9OP9

Keywords:

DE NOVO PROTEIN

Title:

Two Component Protein Nano-Particle (T=3). De Novo Design, Computationally Relaxed into Low Resolution Subtomogram Averaged CryoEM Map with Icosahedral Symmetry Applied

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli 'bl21-gold(de3)plyss ag'

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

31.60 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SUBTOMOGRAM AVERAGING

Download Validation Report

Macromolecular Entities

Protein Blast

Polymer Type:polypeptide(L)
Molecule:C3-A
Chain IDs:C (auth: YD), E (auth: ZZ), G (auth: YF), I (auth: YH), K (auth: YJ), M (auth: YL), O (auth: YN), Q (auth: YP), S (auth: YR), U (auth: YT), W (auth: YV), Y (auth: AB), AA (auth: YX), BA (auth: ZB), DA (auth: YZ), FA (auth: AD), HA (auth: AF), JA (auth: AH), LA (auth: AJ), NA (auth: AL), PA (auth: AN), QA (auth: AO), SA (auth: AQ), VA (auth: AT), XA (auth: AV), ZA (auth: AX), BB (auth: AZ), EB (auth: BB), GB (auth: BD), IB (auth: BF), KB (auth: BH), MB (auth: BJ), OB (auth: BL), QB (auth: BN), SB (auth: BP), UB (auth: BR), WB (auth: BT), YB (auth: BV), AC (auth: BX), CC (auth: BZ), DC (auth: ZD), FC (auth: CB), HC (auth: CD), JC (auth: CF), LC (auth: CH), NC (auth: CJ), PC (auth: CL), RC (auth: CN), TC (auth: CP), VC (auth: CR), XC (auth: CT), ZC (auth: CV), BD (auth: CX), DD (auth: CZ), GD (auth: DB), ID (auth: DD), KD (auth: DF), MD (auth: DH), OD (auth: DJ), QD (auth: DL), SD (auth: DN), UD (auth: DP), WD (auth: DR), YD (auth: DT), AE (auth: DW), CE (auth: DY), EE (auth: EA), FE (auth: ZF), HE (auth: EC), JE (auth: EE), LE (auth: EG), NE (auth: EI), PE (auth: EK), RE (auth: EM), TE (auth: EO), VE (auth: EQ), XE (auth: ES), ZE (auth: EU), BF (auth: EW), DF (auth: EY), FF (auth: FA), IF (auth: FC), KF (auth: FE), MF (auth: FG), OF (auth: FI), QF (auth: FK), SF (auth: FM), UF (auth: FO), WF (auth: FQ), YF (auth: FS), AG (auth: FU), CG (auth: FW), EG (auth: FY), GG (auth: GA), HG (auth: ZH), JG (auth: GC), LG (auth: GE), NG (auth: GG), PG (auth: GI), RG (auth: GK), TG (auth: GM), VG (auth: GO), XG (auth: GQ), ZG (auth: GS), BH (auth: GU), DH (auth: GW), FH (auth: GY), HH (auth: HA), KH (auth: HC), MH (auth: HE), NH (auth: HF), QH (auth: HH), SH (auth: HJ), TH (auth: HK), WH (auth: HM), YH (auth: HO), AI (auth: HQ), CI (auth: HS), EI (auth: HU), GI (auth: HW), II (auth: HY), KI (auth: IA), LI (auth: ZJ), MI (auth: IB), PI (auth: ID), RI (auth: IF), SI (auth: IG), VI (auth: IJ), XI (auth: IL), ZI (auth: IN), BJ (auth: IP), DJ (auth: IR), FJ (auth: IT), HJ (auth: IV), JJ (auth: IX), LJ (auth: IZ), OJ (auth: JB), QJ (auth: JD), SJ (auth: JF), UJ (auth: JH), WJ (auth: JJ), YJ (auth: JL), AK (auth: JN), CK (auth: JP), EK (auth: JR), GK (auth: JT), HK (auth: JU), KK (auth: JW), MK (auth: JY), OK (auth: KA), PK (auth: ZL), RK (auth: KC), TK (auth: KE), VK (auth: KG), XK (auth: KI), ZK (auth: KK), BL (auth: KM), DL (auth: KO), FL (auth: KQ), HL (auth: KS), JL (auth: KU), LL (auth: KW), NL (auth: KY), PL (auth: LA), SL (auth: LC), UL (auth: LE), WL (auth: LG), YL (auth: LI), AM (auth: LK), CM (auth: LM), EM (auth: LO), GM (auth: LQ), IM (auth: LS), JM (auth: ZN), LM (auth: ZP), NM (auth: ZR), PM (auth: ZT), RM (auth: ZV), TM (auth: ZX), VM (auth: YB)
Chain Length:300
Number of Molecules:180
Biological Source:synthetic construct

Protein Blast

Polymer Type:polypeptide(L)
Molecule:C2-B
Chain IDs:A (auth: ZA), B (auth: YC), D (auth: ZY), F (auth: YE), H (auth: YG), J (auth: YI), L (auth: YK), N (auth: YM), P (auth: YO), R (auth: YQ), T (auth: YS), V (auth: YU), X (auth: AA), Z (auth: YW), CA (auth: YY), EA (auth: AC), GA (auth: AE), IA (auth: AG), KA (auth: AI), MA (auth: AK), OA (auth: AM), RA (auth: AP), TA (auth: AR), UA (auth: AS), WA (auth: AU), YA (auth: AW), AB (auth: AY), CB (auth: ZC), DB (auth: BA), FB (auth: BC), HB (auth: BE), JB (auth: BG), LB (auth: BI), NB (auth: BK), PB (auth: BM), RB (auth: BO), TB (auth: BQ), VB (auth: BS), XB (auth: BU), ZB (auth: BW), BC (auth: BY), EC (auth: CA), GC (auth: CC), IC (auth: CE), KC (auth: CG), MC (auth: CI), OC (auth: CK), QC (auth: CM), SC (auth: CO), UC (auth: CQ), WC (auth: CS), YC (auth: CU), AD (auth: CW), CD (auth: CY), ED (auth: ZE), FD (auth: DA), HD (auth: DC), JD (auth: DE), LD (auth: DG), ND (auth: DI), PD (auth: DK), RD (auth: DM), TD (auth: DO), VD (auth: DQ), XD (auth: DS), ZD (auth: DU), BE (auth: DX), DE (auth: DZ), GE (auth: EB), IE (auth: ED), KE (auth: EF), ME (auth: EH), OE (auth: EJ), QE (auth: EL), SE (auth: EN), UE (auth: EP), WE (auth: ER), YE (auth: ET), AF (auth: EV), CF (auth: EX), EF (auth: EZ), GF (auth: ZG), HF (auth: FB), JF (auth: FD), LF (auth: FF), NF (auth: FH), PF (auth: FJ), RF (auth: FL), TF (auth: FN), VF (auth: FP), XF (auth: FR), ZF (auth: FT), BG (auth: FV), DG (auth: FX), FG (auth: FZ), IG (auth: GB), KG (auth: GD), MG (auth: GF), OG (auth: GH), QG (auth: GJ), SG (auth: GL), UG (auth: GN), WG (auth: GP), YG (auth: GR), AH (auth: GT), CH (auth: GV), EH (auth: GX), GH (auth: GZ), IH (auth: ZI), JH (auth: HB), LH (auth: HD), OH (auth: WB), PH (auth: HG), RH (auth: HI), UH (auth: WA), VH (auth: HL), XH (auth: HN), ZH (auth: HP), BI (auth: HR), DI (auth: HT), FI (auth: HV), HI (auth: HX), JI (auth: HZ), NI (auth: WC), OI (auth: IC), QI (auth: IE), TI (auth: IH), UI (auth: II), WI (auth: IK), YI (auth: IM), AJ (auth: IO), CJ (auth: IQ), EJ (auth: IS), GJ (auth: IU), IJ (auth: IW), KJ (auth: IY), MJ (auth: JA), NJ (auth: ZK), PJ (auth: JC), RJ (auth: JE), TJ (auth: JG), VJ (auth: JI), XJ (auth: JK), ZJ (auth: JM), BK (auth: JO), DK (auth: JQ), FK (auth: JS), IK (auth: WD), JK (auth: JV), LK (auth: JX), NK (auth: JZ), QK (auth: KB), SK (auth: KD), UK (auth: KF), WK (auth: KH), YK (auth: KJ), AL (auth: KL), CL (auth: KN), EL (auth: KP), GL (auth: KR), IL (auth: KT), KL (auth: KV), ML (auth: KX), OL (auth: KZ), QL (auth: ZM), RL (auth: LB), TL (auth: LD), VL (auth: LF), XL (auth: LH), ZL (auth: LJ), BM (auth: LL), DM (auth: LN), FM (auth: LP), HM (auth: LR), KM (auth: ZO), MM (auth: ZQ), OM (auth: ZS), QM (auth: ZU), SM (auth: ZW), UM (auth: YA)
Chain Length:114
Number of Molecules:180
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

De novo design of quasisymmetric two-component protein cages

Wang, S, Xie, Y, Chemielewski, D, Weidle, C, Hernandez, C, Chen, W, Ahn, G, Duran, D.C, Bera, A.K, Bethel, N, Decarreau, J, Tong, S, Kang, A, Brackenbrough, E, Joyce, E, Lee, S, Wu, K, Borst, A.J, Favor, A, Buwei, H, DiMaio, F, Holt, L, Baker, D.Show

Nature ? ? ? (2026)

DOI: 10.1038/s41586-026-10464-0

Primary Citation of related structures

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