ISDA: 9NWN

Deposition Date 2025-03-24

Release Date 2025-08-27

Last Version Date 2026-03-11

Entry Detail

PDB ID:

9NWN

Keywords:

IMMUNE SYSTEM

Title:

Structure of minimal CRISPR polymerase bound to ApNHpp

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli bl21

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.28 Å

R-Value Free:

0.24

R-Value Work:

0.22

R-Value Observed:

0.22

Space Group:

P 21 21 2

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:MCpol domain-containing prote
Gene (Uniprot):DU321_04150
Chain IDs:A (auth: B), B (auth: A)
Chain Length:125
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:A0A426EXS8 Pfam ID:PF18182

Ligand Molecules

ZAN

Primary Citation

A miniature CRISPR-Cas10 enzyme confers immunity by inhibitory signalling.

Doherty, E.E, Adler, B.A, Yoon, P.H, Hsieh, K, Loi, K, Armbruster, E.G, Lahiri, A, Bolling, C.S, Wilcox, X.E, Akkati, A, Iavarone, A.T, Pogliano, J, Doudna, J.A.Show

Nature 647 997 1004 (2025)

PMID: 41034576 DOI: 10.1038/s41586-025-09569-9

Primary Citation of related structures

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