ISDA: 9N9Q

Deposition Date 2025-02-11

Release Date 2025-12-24

Last Version Date 2026-03-18

Entry Detail

PDB ID:

9N9Q

Keywords:

RNA BINDING PROTEIN/DNA/RNA

Title:

Cryo-EM structure of the RNA-guided transcription complex

Biological Source:

Source Organism(s):

Flagellimonas taeanensis (Taxon ID: 1005926)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.88 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nuclease-deactivated Cas12f
Chain IDs:A, B
Chain Length:99
Number of Molecules:2
Biological Source:Flagellimonas taeanensis

UniProt ID:A0ABU6IR94

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:RpoE
Chain IDs:C
Chain Length:261
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

UniProt ID:A0ABU6IR18

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:D, E
Chain Length:330
Number of Molecules:2
Biological Source:Flagellimonas taeanensis

UniProt ID:A0A1M6YWR9 Pfam ID:PF01193, PF01000, PF03118

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:F
Chain Length:1269
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

UniProt ID:A0A3A1NXR2 Pfam ID:PF04563, PF04561, PF04565, PF10385, PF00562, PF04560

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:G
Chain Length:1432
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

UniProt ID:A0A3A1NXQ1 Pfam ID:PF04997, PF00623, PF04983, PF05000, PF04998

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA polymerase Rpb6
Gene (Uniprot):SAMN04487891_112109, SAMN05216293_3853
Chain IDs:H
Chain Length:108
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

UniProt ID:A0A1M7BX03 Pfam ID:PF01192

Polymer Type:polydeoxyribonucleotide
Molecule:DNA non-template strand
Chain IDs:I (auth: a)
Chain Length:99
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Polymer Type:polydeoxyribonucleotide
Molecule:DNA template strand
Chain IDs:J (auth: b)
Chain Length:99
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Polymer Type:polyribonucleotide
Molecule:gRNA (85-MER)
Chain IDs:K (auth: c)
Chain Length:261
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Ligand Molecules

Primary Citation

Structural basis of RNA-guided transcription by a dCas12f-sigma E -RNAP complex.

Xiao, R, Hoffmann, F.T, Xie, D, Wiegand, T, Palmieri, A.I, Sternberg, S.H, Chang, L.Show

Nature ? ? ? (2026)

PMID: 41781609 DOI: 10.1038/s41586-026-10178-3

Primary Citation of related structures

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