ISDA: 9N9M

Deposition Date 2025-02-11

Release Date 2025-12-24

Last Version Date 2026-03-18

Entry Detail

PDB ID:

9N9M

Keywords:

RNA BINDING PROTEIN/DNA/RNA

Title:

Cryo-EM structure of the dCas12f-gRNA-DNA complex (partial R-Loop)

Biological Source:

Source Organism(s):

Flagellimonas taeanensis (Taxon ID: 1005926)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.38 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nuclease-deactivated Cas12f
Chain IDs:A, B
Chain Length:60
Number of Molecules:2
Biological Source:Flagellimonas taeanensis

UniProt ID:A0ABU6IR94

Polymer Type:polydeoxyribonucleotide
Molecule:DNA non-template strand
Chain IDs:C (auth: a)
Chain Length:60
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Polymer Type:polydeoxyribonucleotide
Molecule:DNA template strand
Chain IDs:D (auth: b)
Chain Length:60
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Polymer Type:polyribonucleotide
Molecule:gRNA (76-MER)
Chain IDs:E (auth: c)
Chain Length:93
Number of Molecules:1
Biological Source:Flagellimonas taeanensis

Ligand Molecules

Primary Citation

Structural basis of RNA-guided transcription by a dCas12f-sigma E -RNAP complex.

Xiao, R, Hoffmann, F.T, Xie, D, Wiegand, T, Palmieri, A.I, Sternberg, S.H, Chang, L.Show

Nature ? ? ? (2026)

PMID: 41781609 DOI: 10.1038/s41586-026-10178-3

Primary Citation of related structures

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