ISDA: 9N6X

Deposition Date 2025-02-05

Release Date 2025-11-05

Last Version Date 2025-12-24

Entry Detail

PDB ID:

9N6X

Keywords:

RIBOSOME

Title:

SSU processome maturation and disassembly, State B

Biological Source:

Source Organism(s):

Saccharomyces cerevisiae S288C (Taxon ID: 559292)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.71 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polyribonucleotide
Molecule:5'ETS rRNA
Chain IDs:A (auth: L0)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polyribonucleotide
Molecule:18S rRNA
Chain IDs:B (auth: L1)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polyribonucleotide
Molecule:TPA: Saccharomyces cerevisiae
Chain IDs:C (auth: L2)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S18-A
Gene (Uniprot):RPS18A
Chain IDs:D (auth: L3)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX55 Pfam ID:PF00416

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S4-A
Gene (Uniprot):RPS4A
Chain IDs:E (auth: L4)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX35 Pfam ID:PF08071, PF01479, PF00900, PF00467, PF16121

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S5
Gene (Uniprot):RPS5
Chain IDs:F (auth: L5)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P26783 Pfam ID:PF00177

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S6-A
Gene (Uniprot):RPS6A
Chain IDs:G (auth: L6)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX37 Pfam ID:PF01092

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S7-A
Gene (Uniprot):RPS7A
Chain IDs:H (auth: L7)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P26786 Pfam ID:PF01251

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S8-A
Gene (Uniprot):RPS8A
Chain IDs:I (auth: L8)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX39 Pfam ID:PF01201

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S9-A
Gene (Uniprot):RPS9A
Chain IDs:J (auth: L9)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:O13516 Pfam ID:PF00163, PF01479

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S16-A
Gene (Uniprot):RPS16A
Chain IDs:K (auth: LC)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX51 Pfam ID:PF00380

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S11-A
Gene (Uniprot):RPS11A
Chain IDs:L (auth: LD)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX47 Pfam ID:PF16205, PF00366

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S22-A
Gene (Uniprot):RPS22A
Chain IDs:M (auth: LE)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0C0W1 Pfam ID:PF00410

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S24-A
Gene (Uniprot):RPS24A
Chain IDs:N (auth: LF)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX31 Pfam ID:PF01282

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S28-A
Gene (Uniprot):RPS28A
Chain IDs:O (auth: LG)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q3E7X9 Pfam ID:PF01200

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NET1-associated nuclear prote
Gene (Uniprot):NAN1
Chain IDs:P (auth: LH)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q02931 Pfam ID:PF00400

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP8
Chain IDs:Q (auth: LI)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P53276 Pfam ID:PF10395, PF22542

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP15
Chain IDs:R (auth: LJ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q04305 Pfam ID:PF00400, PF09384

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP9
Chain IDs:S (auth: LK)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P38882

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP5
Chain IDs:T (auth: LL)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q04177 Pfam ID:PF04003

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP10
Chain IDs:U (auth: LM)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P42945 Pfam ID:PF12397, PF08146, PF23243

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP4
Chain IDs:V (auth: LN)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06679 Pfam ID:PF00400

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Periodic tryptophan protein 2
Gene (Uniprot):PWP2
Chain IDs:W (auth: LO)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P25635 Pfam ID:PF00400, PF04003

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP6
Chain IDs:X (auth: LP)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q02354 Pfam ID:PF08640, PF05843, PF23240

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):DIP2
Chain IDs:Y (auth: LQ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q12220 Pfam ID:PF00400, PF04003

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP13
Chain IDs:Z (auth: LR)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q05946 Pfam ID:PF00400, PF08625

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP18
Chain IDs:AA (auth: LS)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P40362 Pfam ID:PF00400

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP21
Chain IDs:BA (auth: LT)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06078 Pfam ID:PF00400, PF04192

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Protein SOF1
Gene (Uniprot):SOF1
Chain IDs:CA (auth: LU)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P33750 Pfam ID:PF00400, PF04158

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosome biogenesis protein E
Gene (Uniprot):ENP2
Chain IDs:DA (auth: LV)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P48234 Pfam ID:PF08159, PF23098, PF23097

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP7
Chain IDs:EA (auth: LW)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P40055 Pfam ID:PF00400, PF08149

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA cytidine acetyltransferas
Gene (Uniprot):KRE33
Chain IDs:FA (auth: LX), GA (auth: LY)
Chain Length:0
Number of Molecules:2
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P53914 Pfam ID:PF08351, PF05127, PF13718, PF13725 EC:2.3.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar ribonucleo
Gene (Uniprot):IMP3
Chain IDs:HA (auth: LZ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P32899 Pfam ID:PF00163, PF01479

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Chain IDs:IA (auth: NA)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Something about silencing pro
Gene (Uniprot):SAS10
Chain IDs:JA (auth: NB)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q12136 Pfam ID:PF04000, PF09368

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar ribonucleo
Gene (Uniprot):LCP5
Chain IDs:KA (auth: NC)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P40079 Pfam ID:PF04000

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Bud site selection protein 21
Gene (Uniprot):BUD21
Chain IDs:LA (auth: ND)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q08492 Pfam ID:PF08297

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Protein FAF1
Gene (Uniprot):FAF1
Chain IDs:MA (auth: NE)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P40546

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S13
Gene (Uniprot):RPS13
Chain IDs:NA (auth: NF)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P05756 Pfam ID:PF08069, PF00312

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S14-A
Gene (Uniprot):RPS14A
Chain IDs:OA (auth: NG)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P06367 Pfam ID:PF00411

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP22
Chain IDs:PA (auth: NH)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P53254 Pfam ID:PF03813, PF17403, PF17404, PF17405, PF17406, PF17407

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosomal RNA-processing prot
Gene (Uniprot):RRP7
Chain IDs:QA (auth: NI)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P25368 Pfam ID:PF17799, PF12923

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:KRR1 small subunit processome
Gene (Uniprot):KRR1
Chain IDs:RA (auth: NK)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P25586 Pfam ID:PF17903, PF21800

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS1A
Chain IDs:SA (auth: NM)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P33442 Pfam ID:PF01015

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Protein BFR2
Gene (Uniprot):BFR2
Chain IDs:TA (auth: NN)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06631 Pfam ID:PF13339, PF08164

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S27-A
Gene (Uniprot):RPS27A
Chain IDs:UA (auth: NQ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P35997 Pfam ID:PF01667

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:rRNA biogenesis protein RRP5
Gene (Uniprot):RRP5
Chain IDs:VA (auth: OA)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q05022 Pfam ID:PF00575, PF05843, PF23240

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nucleolar protein 56
Gene (Uniprot):NOP56
Chain IDs:WA (auth: SA)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q12460 Pfam ID:PF08156, PF01798

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nucleolar protein 58
Gene (Uniprot):NOP58
Chain IDs:XA (auth: SB)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q12499 Pfam ID:PF08156, PF01798

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:rRNA 2'-O-methyltransferase f
Gene (Uniprot):NOP1
Chain IDs:YA (auth: SC), ZA (auth: SD)
Chain Length:0
Number of Molecules:2
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P15646 Pfam ID:PF01269 EC:2.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:13 kDa ribonucleoprotein-asso
Gene (Uniprot):SNU13
Chain IDs:AB (auth: SE), BB (auth: SF)
Chain Length:0
Number of Molecules:2
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P39990 Pfam ID:PF01248

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosomal RNA-processing prot
Gene (Uniprot):RRP9
Chain IDs:CB (auth: SG)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06506 Pfam ID:PF00400

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA 3'-terminal phosphate cyc
Gene (Uniprot):RCL1
Chain IDs:DB (auth: SH)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q08096 Pfam ID:PF01137, PF05189

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosome biogenesis protein B
Gene (Uniprot):BMS1
Chain IDs:EB (auth: SI)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q08965 Pfam ID:PF00004, PF08142, PF04950

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosomal RNA small subunit m
Gene (Uniprot):EMG1
Chain IDs:FB (auth: SJ), GB (auth: SK)
Chain Length:0
Number of Molecules:2
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06287 Pfam ID:PF03587 EC:2.1.1.260

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:rRNA-processing protein FCF1
Gene (Uniprot):FCF1
Chain IDs:HB (auth: SL)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q05498 Pfam ID:PF04900

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar ribonucleo
Gene (Uniprot):IMP4
Chain IDs:IB (auth: SM)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P53941 Pfam ID:PF04427

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ribosome biogenesis protein U
Gene (Uniprot):UTP30
Chain IDs:JB (auth: SN)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P36144 Pfam ID:PF00687

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP20
Chain IDs:KB (auth: SP)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P35194 Pfam ID:PF07539, PF20416, PF23099

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:rRNA-processing protein FCF2
Gene (Uniprot):FCF2
Chain IDs:LB (auth: SQ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q12035 Pfam ID:PF08698

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S23-A
Gene (Uniprot):RPS23A
Chain IDs:MB (auth: SR)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P0CX29 Pfam ID:PF00164

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP14
Chain IDs:NB (auth: SS)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q04500 Pfam ID:PF04615

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nucleolar complex protein 14
Gene (Uniprot):NOP14
Chain IDs:OB (auth: ST)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q99207 Pfam ID:PF04147

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Nucleolar complex protein 4
Gene (Uniprot):NOC4
Chain IDs:PB (auth: SU)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q06512 Pfam ID:PF03914

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Regulator of rDNA transcripti
Gene (Uniprot):RRT14
Chain IDs:QB (auth: SV)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P40470 Pfam ID:PF17075

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Pre-rRNA-processing protein P
Gene (Uniprot):PNO1
Chain IDs:RB (auth: SW)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:Q99216 Pfam ID:PF17903, PF22891

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:U3 small nucleolar RNA-associ
Gene (Uniprot):UTP11
Chain IDs:SB (auth: SY)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P34247 Pfam ID:PF03998

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Essential nuclear protein 1
Gene (Uniprot):ENP1
Chain IDs:TB (auth: SZ)
Chain Length:0
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

UniProt ID:P38333 Pfam ID:PF05291

Modified Residue

Compound ID	Chain ID	Parent Comp ID	Details	2D Image
SEP	W	SER	modified residue

Ligand Molecules

ATP

GTP

Primary Citation

Helicase-mediated mechanism of SSU processome maturation and disassembly.

Buzovetsky, O, Klinge, S.Show

Nature 648 746 754 (2025)

PMID: 41162712 DOI: 10.1038/s41586-025-09688-3

Abstact

Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 small nucleolar RNA (snoRNA). The underlying molecular mechanisms of SSU processome maturation, remodelling, disassembly and RNA quality control, and the transitions between states remain unknown owing to a paucity of intermediates1-3. Here we report 16 native SSU processome structures alongside genetic data, revealing how two helicases, the Mtr4-exosome and Dhr1, are controlled for accurate and unidirectional ribosome biogenesis. Our data show how irreversible pre-ribosomal RNA degradation by the redundantly tethered RNA exosome couples the transformation of the SSU processome into a pre-40S particle, during which Utp14 can probe evolving surfaces, ultimately positioning and activating Dhr1 to unwind the U3 snoRNA and initiate nucleolar pre-40S release. This study highlights a paradigm for large dynamic RNA-protein complexes in which irreversible RNA degradation drives compositional changes and communicates these changes to govern enzyme activity while maintaining overall quality control.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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