9LKJ image
Deposition Date 2025-01-16
Release Date 2026-01-21
Last Version Date 2026-08-05
Entry Detail
PDB ID:
9LKJ
Keywords:
IMMUNE SYSTEM
Title:
Structure of Cas9-sgRNA-A27
Biological Source:
Source Organism(s):
Streptococcus pyogenes (Taxon ID: 1314)
Streptococcus pyogenes serotype M1 (Taxon ID: 301447)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.68 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9LKJ validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Phage protein
Gene (Uniprot):FGO82_05925, SAMEA1711581_00005
Chain IDs:A
Chain Length:79
Number of Molecules:1
Biological Source:Streptococcus pyogenes
UniProt ID:A0A4U9C0Y2
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas9
Chain IDs:B (auth: C)
Chain Length:1368
Number of Molecules:1
Biological Source:Streptococcus pyogenes serotype M1
UniProt ID:Q99ZW2 Pfam ID:PF16593, PF16592, PF13395, PF22702, PF16595 EC:3.1.-.-
Polymer Type:polyribonucleotide
Molecule:RNA (98-MER)
Chain IDs:C (auth: D)
Chain Length:92
Number of Molecules:1
Biological Source:Streptococcus pyogenes
Ligand Molecules
NA
Primary Citation
An anti-CRISPR targets the sgRNA to block Cas9 and guides the design of enhanced genome editors.
Nat.Struct.Mol.Biol. 33 318 329 (2026)
PMID: 41555077 DOI: 10.1038/s41594-025-01741-z
Primary Citation of related structures
9LKJ
Feedback Form
Name
Email
Institute
Feedback