ISDA: 9K8R

Deposition Date 2024-10-24

Release Date 2025-02-12

Last Version Date 2025-02-12

Entry Detail

PDB ID:

9K8R

Keywords:

BIOSYNTHETIC PROTEIN

Title:

Structure of Promiscuous short chain dehydrogenase Spm14 in complex with NADPH

Biological Source:

Source Organism(s):

Spiromastix sp. SCSIO F190 (Taxon ID: 2695271)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.10 Å

R-Value Free:

0.20

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

P 32 1 2

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Spm14
Chain IDs:A, B
Chain Length:293
Number of Molecules:2
Biological Source:Spiromastix sp. SCSIO F190

UniProt ID:A0A8J9S3B0 Pfam ID:PF13561

Ligand Molecules

NAP

TRS

Primary Citation

Enzymatic Insights into the Unusual Formation of Benzolactone and Benzopyran in the Biosynthesis of Spiromarmycin

Chen, Y, Jing, L, Peng, M, Cai, C, Shi, J, Ge, W, Liu, Y, Shang, Z, Ma, J, Wu, D, Ju, J.Show

Acs Catalysis 15 2809 2821 (2025)

DOI: 10.1021/acscatal.4c07664

Primary Citation of related structures

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