ISDA: 9IFB

Deposition Date 2025-02-17

Release Date 2026-03-04

Last Version Date 2026-09-16

Entry Detail

PDB ID:

9IFB

Keywords:

HYDROLASE

Title:

Crystal structure of S-adenosyl-L-homocysteine hydrolase mutant C221W from Pyrococcus furiosus in complex with inosine

Biological Source:

Source Organism(s):

Pyrococcus furiosus (Taxon ID: 2261)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.53 Å

R-Value Free:

0.21

R-Value Work:

0.15

Space Group:

P 42 21 2

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Adenosylhomocysteinase
Gene (Uniprot):ahcY
Mutagens:C221W
Chain IDs:A, B
Chain Length:0
Number of Molecules:2
Biological Source:Pyrococcus furiosus

UniProt ID:P50251 Pfam ID:PF05221, PF00670 EC:3.3.1.1

Ligand Molecules

NOS

Primary Citation

S-Adenosyl-l-homocysteine Hydrolase Side Reactivity Enables Biocatalytic Access to 4′,5′-Dehydro Nucleosides as Nucleoside Drug Precursors

Koeppl, L.H, Germer, P, Wolff, T.L, Popadic, D, Bencic, P, Saleem-Batcha, R, Bisel, P, Andexer, J.N.Show

Acs Catalysis 16 15920 15930 (2026)

DOI: 10.1021/acscatal.6c03605

Primary Citation of related structures

Feedback Form