ISDA: 9HLN

Deposition Date 2024-12-05

Release Date 2026-06-17

Last Version Date 2026-06-17

Entry Detail

PDB ID:

9HLN

Keywords:

TRANSFERASE

Title:

Crystal structure of MnmA D100C mutant from Streptococcus pneumoniae with [4Fe-4S] cluster in complex with formate

Biological Source:

Source Organism(s):

Streptococcus pneumoniae TIGR4 (Taxon ID: 170187)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.92 Å

R-Value Free:

0.18

R-Value Work:

0.16

R-Value Observed:

0.16

Space Group:

P 32 2 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:tRNA-specific 2-thiouridylase
Gene (Uniprot):mnmA
Mutagens:D100C
Chain IDs:A
Chain Length:375
Number of Molecules:1
Biological Source:Streptococcus pneumoniae TIGR4

UniProt ID:Q97T38 Pfam ID:PF03054, PF20259, PF20258 EC:2.8.1.13

Ligand Molecules

FMT

GOL

SF4

Primary Citation

A [4Fe-4S] cluster, coordinated by two conserved cysteines and one aspartate, is essential for tRNA thiolation by MnmA enzymes from two Gram positive bacteria

Golinelli-Pimpaneau, B.Show

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Primary Citation of related structures

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