9HCC image
Deposition Date 2024-11-08
Release Date 2025-06-18
Last Version Date 2025-07-16
Entry Detail
PDB ID:
9HCC
Keywords:
RIBOSOME
Title:
Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, State A1 (SAMC knock-out)
Biological Source:
Source Organism(s):
Mus musculus (Taxon ID: 10090)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
5.67 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9HCC validation report
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl32
Chain IDs:W (auth: 0)
Chain Length:187
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9DCI9 Pfam ID:PF01783
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl37
Chain IDs:X (auth: 5)
Chain Length:423
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q921S7 Pfam ID:PF07147
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl38
Chain IDs:Y (auth: 6)
Chain Length:380
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8K2M0 Pfam ID:PF01161
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl39
Chain IDs:Z (auth: 7)
Chain Length:336
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9JKF7
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl40
Chain IDs:AA (auth: 8)
Chain Length:206
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9Z2Q5 Pfam ID:PF09812
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl41
Chain IDs:BA (auth: 9)
Chain Length:135
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQN7 Pfam ID:PF09809
Polymer Type:polyribonucleotide
Molecule:16S rRNA (1584-MER)
Chain IDs:A
Chain Length:142
Number of Molecules:1
Biological Source:Mus musculus
Polymer Type:polyribonucleotide
Molecule:tRNA-Phe (68-MER)
Chain IDs:B
Chain Length:68
Number of Molecules:1
Biological Source:Mus musculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl2
Chain IDs:C (auth: D)
Chain Length:246
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D773 Pfam ID:PF00181, PF03947
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl3
Chain IDs:D (auth: E)
Chain Length:283
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q99N95 Pfam ID:PF00297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl4
Chain IDs:E (auth: F)
Chain Length:211
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9DCU6 Pfam ID:PF00573
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl9
Chain IDs:F (auth: H)
Chain Length:159
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q3UK02 Pfam ID:PF01281, PF22078, PF25131
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl10
Chain IDs:G (auth: I)
Chain Length:128
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q3TBW2
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl11
Chain IDs:H (auth: J)
Chain Length:121
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQF0 Pfam ID:PF03946, PF00298
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl13
Chain IDs:I (auth: K)
Chain Length:178
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D1P0 Pfam ID:PF00572
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl14
Chain IDs:J (auth: L)
Chain Length:135
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D1I6 Pfam ID:PF00238
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl15
Chain IDs:K (auth: M)
Chain Length:295
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CPR5 Pfam ID:PF00828
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl17
Chain IDs:L (auth: O)
Chain Length:176
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D8P4 Pfam ID:PF01196
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl18
Chain IDs:M (auth: P)
Chain Length:206
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQL5 Pfam ID:PF00861
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl19
Chain IDs:N (auth: Q)
Chain Length:292
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D338 Pfam ID:PF01245
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl20
Chain IDs:O (auth: R)
Chain Length:149
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQL4 Pfam ID:PF00453
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl21
Chain IDs:P (auth: S)
Chain Length:442
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D1N9 Pfam ID:PF00829
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl22
Chain IDs:Q (auth: T)
Chain Length:206
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8BU88 Pfam ID:PF00237
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl23
Chain IDs:R (auth: U)
Chain Length:146
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:O35972 Pfam ID:PF00276
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl24
Chain IDs:S (auth: V)
Chain Length:216
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQ06 Pfam ID:PF00467, PF17136
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl28, Mrpl32
Chain IDs:T (auth: X)
Chain Length:418
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D1B9,Q9DCI9 Pfam ID:PF01783, PF00830
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl47
Chain IDs:U (auth: Y)
Chain Length:252
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8K2Y7 Pfam ID:PF06984
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl30
Chain IDs:V (auth: Z)
Chain Length:540
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D7N6 Pfam ID:PF00327
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl42
Chain IDs:CA (auth: a)
Chain Length:142
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CPV3 Pfam ID:PF10210
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl43
Chain IDs:DA (auth: b)
Chain Length:68
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q5RL20 Pfam ID:PF05047
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl44
Chain IDs:EA (auth: c)
Chain Length:308
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CY73 Pfam ID:PF22935, PF22892
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl45
Chain IDs:FA (auth: d)
Chain Length:246
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D0Q7 Pfam ID:PF04280
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl46
Chain IDs:GA (auth: e)
Chain Length:283
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9EQI8 Pfam ID:PF11788
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl48
Chain IDs:HA (auth: f)
Chain Length:211
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8JZS9 Pfam ID:PF00338
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl49
Chain IDs:IA (auth: g)
Chain Length:166
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQ40 Pfam ID:PF05046
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl50
Chain IDs:JA (auth: h)
Chain Length:159
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8VDT9 Pfam ID:PF10501
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl51
Chain IDs:KA (auth: i)
Chain Length:128
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CPY1 Pfam ID:PF10244
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl52
Chain IDs:LA (auth: j)
Chain Length:121
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D0Y8 Pfam ID:PF18699
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl53
Chain IDs:MA (auth: k)
Chain Length:178
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D1H8 Pfam ID:PF10780
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl54
Chain IDs:NA (auth: l)
Chain Length:135
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CPW3 Pfam ID:PF08561
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl57
Chain IDs:OA (auth: o)
Chain Length:176
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CQF8 Pfam ID:PF14978
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrpl58
Chain IDs:PA (auth: p)
Chain Length:206
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q8R035 Pfam ID:PF00472
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Gadd45gip1
Chain IDs:QA (auth: q)
Chain Length:292
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CR59 Pfam ID:PF10147
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrps18a
Chain IDs:RA (auth: r)
Chain Length:149
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q99N85 Pfam ID:PF01084
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):Mrps30
Chain IDs:SA (auth: s)
Chain Length:442
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9D0G0 Pfam ID:PF07147
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Mitochondrial assembly of rib
Gene (Uniprot):Malsu1
Chain IDs:TA (auth: u)
Chain Length:146
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CWV0 Pfam ID:PF02410
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Predicted gene, 55359
Gene (Uniprot):Gm55359
Chain IDs:UA (auth: v)
Chain Length:216
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:A0A2I3BQS8 Pfam ID:PF05347
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Acyl carrier protein, mitocho
Gene (Uniprot):Ndufab1
Chain IDs:VA (auth: w)
Chain Length:156
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CR21 Pfam ID:PF00550
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:rRNA methyltransferase 3, mit
Gene (Uniprot):Mrm3
Chain IDs:WA (auth: x), XA (auth: y)
Chain Length:418
Number of Molecules:2
Biological Source:Mus musculus
UniProt ID:Q5ND52 Pfam ID:PF22435, PF00588
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Probable ATP-dependent RNA he
Gene (Uniprot):Ddx28
Chain IDs:YA (auth: z)
Chain Length:540
Number of Molecules:1
Biological Source:Mus musculus
UniProt ID:Q9CWT6 Pfam ID:PF00270, PF00271
Ligand Molecules
FES
ZN
Primary Citation
The mitochondrial methylation potential gates mitoribosome assembly.
Nat Commun 16 5388 5388 (2025)
PMID: 40562754 DOI: 10.1038/s41467-025-60977-x

Abstact

S-adenosylmethionine (SAM) is the principal methyl donor in cells and is essential for mitochondrial gene expression, influencing RNA modifications, translation, and ribosome biogenesis. Using direct long-read RNA sequencing in mouse tissues and embryonic fibroblasts, we show that processing of the mitochondrial ribosomal gene cluster fails in the absence of mitochondrial SAM, leading to an accumulation of unprocessed precursors. Proteomic analysis of ribosome fractions revealed these precursors associated with processing and assembly factors, indicating stalled biogenesis. Structural analysis by cryo-electron microscopy demonstrated that SAM-dependent methylation is required for peptidyl transferase centre formation during mitoribosome assembly. Our findings identify a critical role for SAM in coordinating mitoribosomal RNA processing and large subunit maturation, linking cellular methylation potential to mitochondrial translation capacity.

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Protein

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Disease

Primary Citation of related structures
9HCC
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