ISDA: 9C5K

Deposition Date 2024-06-06

Release Date 2025-08-27

Last Version Date 2026-08-12

Entry Detail

PDB ID:

9C5K

Keywords:

OXIDOREDUCTASE

Title:

Trypanosoma cruzi R19T/K20S/C64Y mutant D-3-hydroxybutyrate dehydrogenase in complex with NADPH and malonate

Biological Source:

Source Organism(s):

Trypanosoma cruzi (Taxon ID: 5693)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.79 Å

R-Value Free:

0.16

R-Value Work:

0.13

R-Value Observed:

0.13

Space Group:

C 1 2 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Hydroxybutyrate dehydrogenase
Gene (Uniprot):C4B63_13g310
Mutagens:R19T, K20S, C64Y
Chain IDs:A, B, C, D
Chain Length:270
Number of Molecules:4
Biological Source:Trypanosoma cruzi

UniProt ID:A0A2V2VPF1 Pfam ID:PF00106 EC:1.1.1.30

Ligand Molecules

ACT

EDO

MLI

NDP

SO4

Primary Citation

The beta 2 alpha B loop determines NAD(P) cofactor specificity and kinetics in trypanosomal D-3-hydroxybutyrate dehydrogenases.

Hashimoto, H, Mawn, I.H, Escobar-Arrillaga, W, Nguyen, L, Madigan, L.A, Antuono, G, Rossy, T, Sojati, J, Mienko, A, Debler, E.W, Palenchar, J.B.Show

J.Mol.Biol. 438 169946 169946 (2026)

PMID: 42456964 DOI: 10.1016/j.jmb.2026.169946

Primary Citation of related structures

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