ISDA: 9BS6

Deposition Date 2024-05-13

Release Date 2025-05-28

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9BS6

Keywords:

HYDROLASE/DNA/RNA

Title:

CryoEM structure of ThermoCas9 in post-cleavage state with a DNA containing NNNNCGA PAM

Biological Source:

Source Organism(s):

Geobacillus thermodenitrificans (Taxon ID: 33940)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.60 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:E (auth: A)
Chain Length:1082
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

UniProt ID:A0A4R1QIZ8 Pfam ID:PF18470, PF13395, PF18541

Polymer Type:polyribonucleotide
Molecule:RNA (114-MER)
Chain IDs:F (auth: B)
Chain Length:149
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (25-MER)
Chain IDs:A (auth: C)
Chain Length:26
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*GP*TP*AP*TP*AP*CP
Chain IDs:B (auth: D)
Chain Length:34
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*AP*GP*CP*TP*TP*CP*
Chain IDs:C (auth: P)
Chain Length:14
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*CP*TP*AP*GP*A)-3'
Chain IDs:D (auth: X)
Chain Length:6
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules