ISDA: 8UIH

Deposition Date 2023-10-10

Release Date 2025-03-19

Last Version Date 2026-07-15

Entry Detail

PDB ID:

8UIH

Keywords:

IMMUNE SYSTEM

Title:

anti-Phosphohistidine Fab hSC44.ck.20 with 3pTza peptide

Biological Source:

Source Organism(s):

Oryctolagus cuniculus (Taxon ID: 9986)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Homo sapiens

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.85 Å

R-Value Free:

0.24

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

C 1 2 1

Download Validation Report

Macromolecular Entities

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:3ptza peptide
Chain IDs:C (auth: D)
Chain Length:9
Number of Molecules:1
Biological Source:synthetic construct

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:hSC44.ck.20 Fab heavy chain
Chain IDs:A (auth: H)
Chain Length:225
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:hSC44.ck.20 Fab light chain
Chain IDs:B (auth: L)
Chain Length:218
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Ligand Molecules

EDO

Primary Citation

Using phage display for rational engineering of a higher-affinity humanized 3' phosphohistidine-specific antibody.

Martyn, G.D, Kalagiri, R, Veggiani, G, Stanfield, R.L, Choudhuri, I, Sala, M, Meisenhelder, J, Chen, C, Biswas, A, Levy, R.M, Lyumkis, D, Wilson, I.A, Hunter, T, Sidhu, S.S.Show

Commun Chem 8 381 381 (2025)

PMID: 41310398 DOI: 10.1038/s42004-025-01768-9

Primary Citation of related structures

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