8OVA image
Deposition Date 2023-04-25
Release Date 2023-11-29
Last Version Date 2025-10-01
Entry Detail
PDB ID:
8OVA
Keywords:
RIBOSOME
Title:
CRYO-EM STRUCTURE OF TRYPANOSOMA BRUCEI PROCYCLIC FORM 80S RIBOSOME : PARENTAL STRAIN
Biological Source:
Source Organism(s):
Trypanosoma brucei brucei (Taxon ID: 5702)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.47 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
8OVA validation report
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S3a
Gene (Uniprot):Tb10.70.3360
Chain IDs:FA (auth: A0)
Chain Length:256
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38BK6 Pfam ID:PF01015
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S4
Gene (Uniprot):Tb11.02.1085, Tb11.02.1090
Chain IDs:GA (auth: A1)
Chain Length:273
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q386F2 Pfam ID:PF08071, PF01479, PF00900, PF00467, PF16121
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S5, put
Gene (Uniprot):Tb11.02.4170
Chain IDs:HA (auth: A2)
Chain Length:190
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q385D9 Pfam ID:PF00177
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S6
Gene (Uniprot):Tb10.100.0080
Chain IDs:IA (auth: A3)
Chain Length:250
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CL9 Pfam ID:PF01092
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S7
Gene (Uniprot):Tb09.160.2550
Chain IDs:JA (auth: A4)
Chain Length:202
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38FD9 Pfam ID:PF01251
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S8
Gene (Uniprot):Tb08.11J15.560
Chain IDs:KA (auth: A5)
Chain Length:220
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57YL6 Pfam ID:PF01201
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Probable 40S ribosomal protei
Chain IDs:LA (auth: A6)
Chain Length:190
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P17959 Pfam ID:PF00163, PF01479
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Guanine nucleotide-binding pr
Chain IDs:MA (auth: A7)
Chain Length:318
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P69103 Pfam ID:PF00400
Polymer Type:polypeptide(L)
Molecule:uS14
Chain IDs:L (auth: A8)
Chain Length:57
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ubiquitin-60S ribosomal prote
Gene (Uniprot):Tb07.28B13.250
Chain IDs:M (auth: A9)
Chain Length:153
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57WI3 Pfam ID:PF00240, PF01599
Polymer Type:polyribonucleotide
Molecule:SSU rRNA
Chain IDs:B (auth: AA)
Chain Length:2282
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:E-SITE TRNA
Chain IDs:J (auth: AB)
Chain Length:19
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein SA
Gene (Uniprot):Tb11.01.2680
Chain IDs:NA (auth: AC)
Chain Length:277
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383I8 Pfam ID:PF00318
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S10, pu
Gene (Uniprot):Tb10.70.1670
Chain IDs:OA (auth: AD)
Chain Length:172
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38B66 Pfam ID:PF03501
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal proteins S11, p
Gene (Uniprot):Tb10.70.7695
Chain IDs:PA (auth: AE)
Chain Length:174
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CI4 Pfam ID:PF16205, PF00366
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S12
Gene (Uniprot):RPS12
Chain IDs:HC (auth: AF)
Chain Length:144
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q03253 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S13, pu
Gene (Uniprot):1F7.325
Chain IDs:QA (auth: AG)
Chain Length:151
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q586C2 Pfam ID:PF08069, PF00312
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S14
Gene (Uniprot):RPS14
Chain IDs:O (auth: AH)
Chain Length:144
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P19800 Pfam ID:PF00411
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S15, pu
Gene (Uniprot):Tb07.22O10.360
Chain IDs:P (auth: AI)
Chain Length:152
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57XN7 Pfam ID:PF00203
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S15a, p
Gene (Uniprot):Tb03.26J7.620
Chain IDs:Q (auth: AJ)
Chain Length:130
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q580K6 Pfam ID:PF00410
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S16, pu
Gene (Uniprot):Tb07.29K4.50
Chain IDs:R (auth: AK)
Chain Length:149
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57VV0 Pfam ID:PF00380
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S17, pu
Gene (Uniprot):Tb11.01.3675, Tb11.01.3676
Chain IDs:EA (auth: AL)
Chain Length:142
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q382Z8 Pfam ID:PF00833
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S18, pu
Gene (Uniprot):Tb10.70.1730, Tb10.70.1740
Chain IDs:S (auth: AM)
Chain Length:153
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38B69 Pfam ID:PF00416
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein S19, putati
Gene (Uniprot):Tb04.29M18.160
Chain IDs:T (auth: AO)
Chain Length:167
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q583K7 Pfam ID:PF01090
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S2, put
Gene (Uniprot):Tb10.61.1960, Tb10.61.2070
Chain IDs:U (auth: AP)
Chain Length:266
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q388D3 Pfam ID:PF00333, PF03719
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein S10
Chain IDs:K (auth: AQ)
Chain Length:117
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S21, pu
Gene (Uniprot):Tb11.02.4360
Chain IDs:V (auth: AR)
Chain Length:194
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q385B8 Pfam ID:PF01249
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S23, pu
Gene (Uniprot):Tb10.70.7020, Tb10.70.7030
Chain IDs:W (auth: AS)
Chain Length:143
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CD1 Pfam ID:PF00164
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S24
Gene (Uniprot):Tb10.6k15.3340, Tb10.6k15.3350
Chain IDs:X (auth: AT)
Chain Length:137
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38AM9 Pfam ID:PF01282
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S25
Gene (Uniprot):Tb03.1J15.510
Chain IDs:Y (auth: AU)
Chain Length:113
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57XX0 Pfam ID:PF03297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S26
Gene (Uniprot):Tb11.01.0355
Chain IDs:Z (auth: AV)
Chain Length:111
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q384J9 Pfam ID:PF01283
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S27, pu
Gene (Uniprot):Tb11.01.1475
Chain IDs:AA (auth: AW)
Chain Length:86
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383V7 Pfam ID:PF01667
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S3, put
Gene (Uniprot):Tb09.160.4450
Chain IDs:BA (auth: AX)
Chain Length:214
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38EW1 Pfam ID:PF07650, PF00189
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S30
Gene (Uniprot):Tb06.4M18.360
Chain IDs:CA (auth: AY)
Chain Length:66
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q584U5 Pfam ID:PF04758
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S33, pu
Gene (Uniprot):Tb07.8P12.320
Chain IDs:DA (auth: AZ)
Chain Length:103
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57U30 Pfam ID:PF01200
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:RNA-binding protein
Gene (Uniprot):Tb11.01.5590
Chain IDs:N (auth: Az)
Chain Length:103
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q382D5 Pfam ID:PF00076
Polymer Type:polyribonucleotide
Molecule:LSUa rRNA
Chain IDs:A (auth: BA)
Chain Length:133
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:LSUb rRNA
Chain IDs:C (auth: BB)
Chain Length:145
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:5.8S rRNA
Chain IDs:I (auth: BC)
Chain Length:146
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:5S rRNA
Chain IDs:H (auth: BD)
Chain Length:71
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:SrRNA-1
Chain IDs:G (auth: BE)
Chain Length:260
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:SrRNA-6
Chain IDs:F (auth: BF)
Chain Length:78
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:SrRNA-2
Chain IDs:E (auth: BG)
Chain Length:105
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polyribonucleotide
Molecule:SrRNA-4
Chain IDs:D (auth: BH)
Chain Length:136
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L18
Gene (Uniprot):RPL18-A, RPL18-B
Chain IDs:AB (auth: BI)
Chain Length:132
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P50885 Pfam ID:PF17135
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L10, pu
Gene (Uniprot):Tb09.211.0110, Tb09.211.0340
Chain IDs:CB (auth: BK)
Chain Length:127
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38EC5 Pfam ID:PF00252
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L11, pu
Gene (Uniprot):Tb09.160.5580, Tb09.160.5590
Chain IDs:EB (auth: BL)
Chain Length:149
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38EI9 Pfam ID:PF00281, PF00673
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L13
Gene (Uniprot):Tb03.3K10.380
Chain IDs:HB (auth: BN)
Chain Length:218
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57V55 Pfam ID:PF01294
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L13a, p
Gene (Uniprot):Tb04.26G5.360
Chain IDs:JB (auth: BO)
Chain Length:222
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q585J5 Pfam ID:PF00572
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein L14, pu
Gene (Uniprot):Tb11.01.3020
Chain IDs:GC (auth: BP)
Chain Length:82
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383F5 Pfam ID:PF00467, PF01929
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L15
Gene (Uniprot):Tb09.244.2720
Chain IDs:NB (auth: BQ)
Chain Length:221
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CY7 Pfam ID:PF00827
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L17, pu
Gene (Uniprot):Tb10.61.2090, Tb11.02.2430
Chain IDs:OB (auth: BR)
Chain Length:374
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q385T6 Pfam ID:PF00237
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L18a
Gene (Uniprot):Tb10.70.3510, Tb11.01.5720
Chain IDs:QB (auth: BS)
Chain Length:128
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q382C4 Pfam ID:PF01775
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L19, pu
Gene (Uniprot):Tb07.27E10.210
Chain IDs:SB (auth: BT)
Chain Length:260
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57ZI7 Pfam ID:PF01280, PF25476
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L21E, p
Gene (Uniprot):Tb11.0880, Tb11.50.0005
Chain IDs:UB (auth: BU)
Chain Length:159
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q4FKD4 Pfam ID:PF01157
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L22, pu
Gene (Uniprot):Tb11.01.1920
Chain IDs:WB (auth: BV)
Chain Length:130
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383R3 Pfam ID:PF01776
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L23, pu
Gene (Uniprot):Tb09.211.2630, Tb09.211.2640
Chain IDs:YB (auth: BW)
Chain Length:257
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38DP2 Pfam ID:PF00238
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L23a
Gene (Uniprot):RPL23A
Chain IDs:ZB (auth: BX)
Chain Length:164
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P41165 Pfam ID:PF00276
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L24, pu
Gene (Uniprot):Tb10.70.1540
Chain IDs:CC (auth: BY)
Chain Length:125
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38B56 Pfam ID:PF01246
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L26, pu
Gene (Uniprot):Tb09.211.4850
Chain IDs:EC (auth: BZ)
Chain Length:143
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38D25 Pfam ID:PF16906, PF00467
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L27
Gene (Uniprot):Tb11.01.7535, Tb11.01.7545
Chain IDs:SA (auth: Ba)
Chain Length:133
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q381J6 Pfam ID:PF00467, PF01777
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L27a
Gene (Uniprot):Tb09.211.2650, Tb09.v1.0640
Chain IDs:TA (auth: Bb)
Chain Length:145
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38DN9 Pfam ID:PF00828
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L28, pu
Gene (Uniprot):Tb11.02.4050, Tb11.02.4070
Chain IDs:UA (auth: Bc)
Chain Length:146
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q385E9 Pfam ID:PF01778
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L29
Gene (Uniprot):Tb11.01.1790
Chain IDs:VA (auth: Bd)
Chain Length:71
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383S6 Pfam ID:PF01779
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L2, put
Gene (Uniprot):Tb05.28F8.80
Chain IDs:WA (auth: Be)
Chain Length:260
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57W56 Pfam ID:PF00181, PF03947
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L3, mitocho
Gene (Uniprot):Tb04.2L9.1340
Chain IDs:ZA (auth: Bf)
Chain Length:78
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q581Q1 Pfam ID:PF00297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L30
Gene (Uniprot):RPL30
Chain IDs:YA (auth: Bg)
Chain Length:105
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P49153 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal subunit protein
Gene (Uniprot):Tb09.211.3270, Tb09.211.3280
Chain IDs:RA (auth: Bh)
Chain Length:136
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38DH8 Pfam ID:PF01198
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L32, pu
Gene (Uniprot):Tb10.100.0155
Chain IDs:FC (auth: Bi)
Chain Length:132
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CL1 Pfam ID:PF01655
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L34, pu
Gene (Uniprot):Tb11.01.1480
Chain IDs:BB (auth: Bj)
Chain Length:170
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383V6 Pfam ID:PF01199
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L35, pu
Gene (Uniprot):Tb09.160.0700, Tb09.160.0710
Chain IDs:DB (auth: Bk)
Chain Length:127
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38FW8 Pfam ID:PF00831
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L35A, p
Gene (Uniprot):Tb04.1H19.150
Chain IDs:FB (auth: Bl)
Chain Length:149
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q584G9 Pfam ID:PF01247
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L36, putati
Gene (Uniprot):Tb09.244.2725
Chain IDs:GB (auth: Bm)
Chain Length:109
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CY6 Pfam ID:PF01158
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L37
Gene (Uniprot):Tb09.211.0865, Tb11.02.0500
Chain IDs:IB (auth: Bn)
Chain Length:218
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q386L1 Pfam ID:PF01907
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L37a, p
Gene (Uniprot):Tb10.100.0110, Tb10.70.0465
Chain IDs:KB (auth: Bo)
Chain Length:222
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38AW9 Pfam ID:PF01780
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L38, pu
Gene (Uniprot):Tb09.160.0815
Chain IDs:LB (auth: Bp)
Chain Length:82
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38FV6 Pfam ID:PF01781
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L39, pu
Gene (Uniprot):Tb08.5H5.400
Chain IDs:MB (auth: Bq)
Chain Length:221
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57UJ8 Pfam ID:PF00832
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L4
Gene (Uniprot):Tb03.48K5.830
Chain IDs:PB (auth: Br)
Chain Length:374
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q582N2 Pfam ID:PF00573, PF14374
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ubiquitin-60S ribosomal prote
Chain IDs:RB (auth: Bs)
Chain Length:128
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P21899 Pfam ID:PF00240, PF01020
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L44
Gene (Uniprot):RPL44
Chain IDs:TB (auth: Bt)
Chain Length:260
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:P17843 Pfam ID:PF00935
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L5, put
Gene (Uniprot):Tb09.244.2730, Tb09.244.2740
Chain IDs:VB (auth: Bu)
Chain Length:159
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CY8 Pfam ID:PF17144, PF14204
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L6, put
Gene (Uniprot):Tb10.26.0560
Chain IDs:XB (auth: Bv)
Chain Length:130
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q389K1 Pfam ID:PF01159
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L7, put
Gene (Uniprot):Tb07.43M14.130
Chain IDs:AC (auth: Bw)
Chain Length:257
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57V35 Pfam ID:PF08079, PF00327
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L7a
Gene (Uniprot):Tb08.29O4.590
Chain IDs:BC (auth: Bx)
Chain Length:164
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57VM1 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L9, put
Gene (Uniprot):Tb10.70.7010
Chain IDs:DC (auth: By)
Chain Length:125
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38CD0 Pfam ID:PF00347
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L41
Chain IDs:XA (auth: Bz)
Chain Length:143
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
A2M I A modified residue
OMG I G modified residue
OMU I U modified residue
PSU I U modified residue
Ligand Molecules
K
MG
NA
ZN
Primary Citation
A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
Nat Commun 14 7462 7462 (2023)
PMID: 37985661 DOI: 10.1038/s41467-023-43263-6

Abstact

Trypanosomes are protozoan parasites that cycle between insect and mammalian hosts and are the causative agent of sleeping sickness. Here, we describe the changes of pseudouridine (Ψ) modification on rRNA in the two life stages of the parasite using four different genome-wide approaches. CRISPR-Cas9 knock-outs of all four snoRNAs guiding Ψ on helix 69 (H69) of the large rRNA subunit were lethal. A single knock-out of a snoRNA guiding Ψ530 on H69 altered the composition of the 80S monosome. These changes specifically affected the translation of only a subset of proteins. This study correlates a single site Ψ modification with changes in ribosomal protein stoichiometry, supported by a high-resolution cryo-EM structure. We propose that alteration in rRNA modifications could generate ribosomes preferentially translating state-beneficial proteins.

Legend

Protein

Chemical

Disease

Primary Citation of related structures
8OVA
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