ISDA: 7SPM

Deposition Date 2021-11-02

Release Date 2021-11-10

Last Version Date 2023-11-01

Entry Detail

PDB ID:

7SPM

Keywords:

DNA BINDING PROTEIN/DNA

Title:

Replication Initiator Protein REPE54 and cognate DNA sequence with terminal three prime phosphates chemically crosslinked (30 mg/mL EDC, 12 hours, 2 doses).

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.28 Å

R-Value Free:

0.25

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

C 1 2 1

Download Validation Report

Macromolecular Entities

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*CP*TP*GP*TP*GP*
Chain IDs:A
Chain Length:22
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*TP*GP*AP*GP*GP*
Chain IDs:B
Chain Length:22
Number of Molecules:1
Biological Source:Escherichia coli

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:RepB family plasmid replicati
Mutagens:R118P
Chain IDs:C
Chain Length:263
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P03856 Pfam ID:PF01051, PF21205

Ligand Molecules

Primary Citation

Stabilizing DNA-Protein Co-Crystals via Intra-Crystal Chemical Ligation of the DNA

Orun, A.R, Dmytriw, S, Vajapayajula, A, Snow, C.D.Show

Crystals 12 ? ? (2022)

DOI: 10.3390/cryst12010049

Primary Citation of related structures

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