7RCF image
Deposition Date 2021-07-07
Release Date 2022-06-29
Last Version Date 2023-10-18
Entry Detail
PDB ID:
7RCF
Keywords:
DNA BINDING PROTEIN/DNA
Title:
Fourth stage reengineered variant of I-OnuI with stability enhancing substitutions
Biological Source:
Source Organism(s):
Synthetic construct (Taxon ID: 32630)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.23 Å
R-Value Free:
0.22
R-Value Work:
0.18
R-Value Observed:
0.19
Space Group:
P 21 21 21
7RCF validation report
Macromolecular Entities
Polymer Type:polypeptide(L)
Molecule:I-OnuI_e-hPD1-e
Chain IDs:A
Chain Length:300
Number of Molecules:1
Biological Source:Synthetic construct
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (26-MER)
Chain IDs:B
Chain Length:26
Number of Molecules:1
Biological Source:Synthetic construct
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (26-MER)
Chain IDs:C
Chain Length:26
Number of Molecules:1
Biological Source:Synthetic construct
Ligand Molecules
CA
CL
EDO
NA
Primary Citation
Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published ? ? ? (?)
Primary Citation of related structures
7RCF
Feedback Form
Name
Email
Institute
Feedback