ISDA: 7PFP

Deposition Date 2021-08-11

Release Date 2022-03-16

Last Version Date 2024-10-16

Entry Detail

PDB ID:

7PFP

Keywords:

ANTIMICROBIAL PROTEIN

Title:

Full-length cryo-EM structure of the native human uromodulin (UMOD)/Tamm-Horsfall protein (THP) filament

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

6.10 Å

Aggregation State:

FILAMENT

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Uromodulin
Gene (Uniprot):UMOD
Chain IDs:A, B, C
Chain Length:640
Number of Molecules:3
Biological Source:Homo sapiens

UniProt ID:P07911 Pfam ID:PF12947, PF07645, PF00100, PF23283, PF23344

Ligand Molecules

Primary Citation

Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.

Stsiapanava, A, Xu, C, Nishio, S, Han, L, Yamakawa, N, Carroni, M, Tunyasuvunakool, K, Jumper, J, de Sanctis, D, Wu, B, Jovine, L.Show

Nat. Struct. Mol. Biol. 29 190 193 (2022)

PMID: 35273390 DOI: 10.1038/s41594-022-00729-3

Abstact

Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast nephropathy.

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Primary Citation of related structures

Abstact

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