ISDA: 7F9O

Deposition Date 2021-07-04

Release Date 2021-12-22

Last Version Date 2026-08-12

Entry Detail

PDB ID:

7F9O

Keywords:

PHOTOSYNTHESIS

Title:

PSI-NDH supercomplex of Barley

Biological Source:

Source Organism(s):

Hordeum vulgare subsp. spontaneum (Taxon ID: 77009)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

4.50 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:NA (auth: 0)
Chain Length:155
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2DWH9

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Chain IDs:L (auth: 1), ZA (auth: p)
Chain Length:247
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Gene (Uniprot):Lhca2
Chain IDs:M (auth: 2)
Chain Length:255
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:Q43485 Pfam ID:PF00504

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Chain IDs:N (auth: 3), AB (auth: q)
Chain Length:269
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2DAN8 Pfam ID:PF00504

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:HA (auth: 4)
Chain Length:130
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Chain IDs:O (auth: 5)
Chain Length:257
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:JA (auth: 6)
Chain Length:469
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2D3G1

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:KA (auth: 7)
Chain Length:361
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2D932

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:LA (auth: 8)
Chain Length:119
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2DD79 Pfam ID:PF00111

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:MA (auth: 9)
Chain Length:83
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I P700 chlorophyl
Gene (Uniprot):psaA
Chain IDs:A, OA (auth: e)
Chain Length:147
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z1K7 Pfam ID:PF00223 EC:1.97.1.12

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I P700 chlorophyl
Gene (Uniprot):psaB
Chain IDs:B, PA (auth: f)
Chain Length:216
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z289 Pfam ID:PF00223 EC:1.97.1.12

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I iron-sulfur cen
Gene (Uniprot):psaC
Chain IDs:C, QA (auth: g)
Chain Length:81
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4YZ47 Pfam ID:PF12838 EC:1.97.1.12

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):psaD
Chain IDs:D, RA (auth: h)
Chain Length:205
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P36213 Pfam ID:PF02531

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):PSAE
Chain IDs:E, SA (auth: i)
Chain Length:147
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P13194 Pfam ID:PF02427

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):PSAF
Chain IDs:F, TA (auth: j)
Chain Length:42
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P13192 Pfam ID:PF02507

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhA
Chain IDs:P (auth: G)
Chain Length:81
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P92432 Pfam ID:PF00146 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):PSAH
Chain IDs:G (auth: H), UA (auth: k)
Chain Length:143
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P20143 Pfam ID:PF03244

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):psaI
Chain IDs:H (auth: I), VA (auth: l)
Chain Length:209
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z1D9 Pfam ID:PF00796

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):psaJ
Chain IDs:I (auth: J), WA (auth: m)
Chain Length:42
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z1E9 Pfam ID:PF01701

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):PSAK
Chain IDs:J (auth: K), XA (auth: n)
Chain Length:143
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P36886 Pfam ID:PF01241

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosystem I reaction center
Gene (Uniprot):PSAL
Chain IDs:K (auth: L), YA (auth: o)
Chain Length:209
Number of Molecules:2
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:P23993 Pfam ID:PF02605

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhB
Chain IDs:Q (auth: M)
Chain Length:42
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z268 Pfam ID:PF19530, PF00361 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhC
Chain IDs:R (auth: N)
Chain Length:117
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z1L1 Pfam ID:PF00507 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhD
Chain IDs:S (auth: O)
Chain Length:209
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z8N2 Pfam ID:PF00361 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhE
Chain IDs:T (auth: P)
Chain Length:247
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z1I1 Pfam ID:PF00420 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NADH-plastoquinone oxidoreduc
Gene (Uniprot):ndhF
Chain IDs:U (auth: Q)
Chain Length:777
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:A0A7T7JN26 Pfam ID:PF00662, PF00361, PF01010

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhG
Chain IDs:V (auth: R)
Chain Length:197
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:A0A4Y5SEJ9 Pfam ID:PF00499 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhH
Chain IDs:W (auth: S)
Chain Length:211
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z272 Pfam ID:PF00346 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhI
Chain IDs:X (auth: T)
Chain Length:165
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:A0A218LNN1 Pfam ID:PF12838 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhJ
Chain IDs:Y (auth: U)
Chain Length:159
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:Q7GT16 Pfam ID:PF00329 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Gene (Uniprot):ndhK
Chain IDs:Z (auth: V)
Chain Length:245
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:S4Z232 Pfam ID:PF01058 EC:7.1.1.-

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Chain IDs:AA (auth: W)
Chain Length:192
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2CUR7 Pfam ID:PF10716

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Chain IDs:BA (auth: X)
Chain Length:213
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2D138 Pfam ID:PF10664

Polymer Type:polypeptide(L)
Molecule:NAD(P)H-quinone oxidoreductas
Chain IDs:CA (auth: Y)
Chain Length:233
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Polymer Type:polypeptide(L)
Molecule:Unidentified stromal protein
Chain IDs:DA (auth: Z)
Chain Length:61
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:EA (auth: a)
Chain Length:750
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:FA (auth: b)
Chain Length:216
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2CTW7 Pfam ID:PF05757

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:GA (auth: c)
Chain Length:81
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Photosynthetic NDH subunit of
Chain IDs:IA (auth: d)
Chain Length:205
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2CTD7 Pfam ID:PF00160

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Gene (Uniprot):Lhca4
Chain IDs:BB (auth: r)
Chain Length:197
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:Q9LKC7 Pfam ID:PF00504

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Chlorophyll a-b binding prote
Chain IDs:CB (auth: s)
Chain Length:211
Number of Molecules:1
Biological Source:Hordeum vulgare subsp. spontaneum

UniProt ID:F2D0D5 Pfam ID:PF00504

Ligand Molecules

BCR

CHL

CL0

CLA

DGD

LHG

LMG

LUT

PQN

SF4

SQD

XAT

Primary Citation

Architecture of the chloroplast PSI-NDH supercomplex in Hordeum vulgare.

Shen, L, Tang, K, Wang, W, Wang, C, Wu, H, Mao, Z, An, S, Chang, S, Kuang, T, Shen, J.R, Han, G, Zhang, X.Show

Nature 601 649 654 (2022)

PMID: 34879391 DOI: 10.1038/s41586-021-04277-6

Abstact

The chloroplast NADH dehydrogenase-like (NDH) complex is composed of at least 29 subunits and has an important role in mediating photosystem I (PSI) cyclic electron transport (CET)1-3. The NDH complex associates with PSI to form the PSI-NDH supercomplex and fulfil its function. Here, we report cryo-electron microscopy structures of a PSI-NDH supercomplex from barley (Hordeum vulgare). The structures reveal that PSI-NDH is composed of two copies of the PSI-light-harvesting complex I (LHCI) subcomplex and one NDH complex. Two monomeric LHCI proteins, Lhca5 and Lhca6, mediate the binding of two PSI complexes to NDH. Ten plant chloroplast-specific NDH subunits are presented and their exact positions as well as their interactions with other subunits in NDH are elucidated. In all, this study provides a structural basis for further investigations on the functions and regulation of PSI-NDH-dependent CET.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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