ISDA: 7EQ9

Deposition Date 2021-04-30

Release Date 2021-09-15

Last Version Date 2023-11-29

Entry Detail

PDB ID:

7EQ9

Keywords:

DE NOVO PROTEIN

Title:

Cryo-EM structure of designed protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.30 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:TIP60
Chain IDs:A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U (auth: V), V (auth: W), W (auth: X), X (auth: Y), Y (auth: Z), Z (auth: AA), AA (auth: BA), BA (auth: CA), CA (auth: DA), DA (auth: EA), EA (auth: FA), FA (auth: GA), GA (auth: HA), HA (auth: IA), IA (auth: JA), JA (auth: KA), KA (auth: LA), LA (auth: MA), MA (auth: NA), NA (auth: OA), OA (auth: PA), PA (auth: QA), QA (auth: RA), RA (auth: SA), SA (auth: TA), TA (auth: UA), UA (auth: VA), VA (auth: WA), WA (auth: XA), XA (auth: YA), YA (auth: ZA), ZA (auth: AB), AB (auth: BB), BB (auth: CB), CB (auth: DB), DB (auth: EB), EB (auth: FB), FB (auth: GB), GB (auth: HB), HB (auth: IB)
Chain Length:153
Number of Molecules:60
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60.

Obata, J, Kawakami, N, Tsutsumi, A, Nasu, E, Miyamoto, K, Kikkawa, M, Arai, R, Kawakami, N, Kondo, H, Matsuzawa, Y, Hayasaka, K, Nasu, E, Sasahara, K, Arai, R, Miyamoto, K.Show

Chem.Commun.(Camb.) 57 10226 10229 (2021)

PMID: 34523636 DOI: 10.1039/d1cc03114g

Abstact

Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications.

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Abstact

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