ISDA: 6W2Z

Deposition Date 2020-03-08

Release Date 2020-03-25

Last Version Date 2024-10-30

Entry Detail

PDB ID:

6W2Z

Keywords:

HYDROLASE

Title:

Crystal Structure of the Beta Lactamase Class A PenP from Bacillus subtilis in the Complex with the Non-beta- lactam Beta-lactamase Inhibitor Avibactam

Biological Source:

Source Organism(s):

Bacillus subtilis (strain 168) (Taxon ID: 224308)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.50 Å

R-Value Free:

0.19

R-Value Work:

0.16

R-Value Observed:

0.16

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Beta-lactamase
Gene (Uniprot):penP
Chain IDs:A, B
Chain Length:275
Number of Molecules:2
Biological Source:Bacillus subtilis (strain 168)

UniProt ID:P39824 Pfam ID:PF00144 EC:3.5.2.6

Ligand Molecules

EDO

FMT

NXL

Primary Citation

Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria.

Inniss, N.L, Minasov, G, Chang, C, Tan, K, Kim, Y, Maltseva, N, Stogios, P, Filippova, E, Michalska, K, Osipiuk, J, Jaroszewki, L, Godzik, A, Savchenko, A, Joachimiak, A, Anderson, W.F, Satchell, K.J.F.Show

Microbiol Resour Announc ? e0020025 e0020025 (2025)

PMID: 40391899 DOI: 10.1128/mra.00200-25

Abstact

Antibiotic resistance remains a leading cause of severe infections worldwide. Small changes in protein sequence can impact antibiotic efficacy. Here, we report deposition of 58 X-ray crystal structures of bacterial proteins that are known targets for antibiotics, which expands knowledge of structural variation to support future antibiotic discovery or modifications.

Legend

Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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