6SG9 image
Deposition Date 2019-08-03
Release Date 2019-09-18
Last Version Date 2025-12-10
Entry Detail
PDB ID:
6SG9
Keywords:
RIBOSOME
Title:
Head domain of the mt-SSU assemblosome from Trypanosoma brucei
Biological Source:
Source Organism(s):
Trypanosoma brucei brucei (Taxon ID: 5702)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.10 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
6SG9 validation report missing
Macromolecular Entities
Polymer Type:polyribonucleotide
Molecule:9S rRNA
Chain IDs:G (auth: CA)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:uS3m
Chain IDs:H (auth: CC)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:uS5m
Chain IDs:A (auth: CE)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:uS9m
Gene (Uniprot):Tb08.28F14.630
Chain IDs:I (auth: CI)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57W62 Pfam ID:PF00380
Polymer Type:polypeptide(L)
Molecule:uS10m
Chain IDs:J (auth: CJ)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:uS11m
Gene (Uniprot):Tb10.406.0510
Chain IDs:B (auth: CK)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q389T7
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:uS14m
Gene (Uniprot):Tb08.29O9.64
Chain IDs:K (auth: CN)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q580I0
Polymer Type:polypeptide(L)
Molecule:uS19m
Chain IDs:L (auth: CS)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS29
Gene (Uniprot):Tb06.3A7.780
Chain IDs:M (auth: Cg)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q585C2 Pfam ID:PF10236
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS33
Gene (Uniprot):Tb03.27F10.720
Chain IDs:N (auth: Ci)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57WW0
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS35
Gene (Uniprot):Tb11.47.0024
Chain IDs:O (auth: Ck)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q387C7
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS38
Gene (Uniprot):Tb07.29K4.740
Chain IDs:C (auth: Cn)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57VQ9
Polymer Type:polypeptide(L)
Molecule:mS49
Chain IDs:P (auth: DB)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:mS50
Chain IDs:Q (auth: DC)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS52
Gene (Uniprot):Tb11.02.0130
Chain IDs:R (auth: DE)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q386Q7
Polymer Type:polypeptide(L)
Molecule:mS53
Chain IDs:S (auth: DF)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:mS54
Chain IDs:T (auth: DG)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:mS55 (KRIPP8)
Chain IDs:U (auth: DH)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS57
Gene (Uniprot):Tb06.4M18.330
Chain IDs:V (auth: DJ)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q584U8
Polymer Type:polypeptide(L)
Molecule:mS58
Chain IDs:W (auth: DK)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS67
Gene (Uniprot):Tb06.30P15.560
Chain IDs:X (auth: DT)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q586G5 Pfam ID:PF00581
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS69
Gene (Uniprot):Tb08.4A8.150
Chain IDs:Y (auth: DV)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57UZ6
Polymer Type:polypeptide(L)
Molecule:mS70
Chain IDs:Z (auth: DW)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS71
Gene (Uniprot):Tb11.01.2920
Chain IDs:AA (auth: DX)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q383G5
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mS72
Gene (Uniprot):Tb07.13M20.50
Chain IDs:BA (auth: DY)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57YD4
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF1 (RSM22)
Gene (Uniprot):Tb11.02.2710
Chain IDs:CA (auth: F1)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q385R2 Pfam ID:PF09243
Polymer Type:polypeptide(L)
Molecule:mt-SAF4
Chain IDs:DA (auth: F4)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF12 (KRIPP18)
Gene (Uniprot):Tb06.3D8.320
Chain IDs:EA (auth: FD)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q583U2
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF14
Gene (Uniprot):Tb08.28F14.650
Chain IDs:FA (auth: FF)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57W60 Pfam ID:PF01795
Polymer Type:polypeptide(L)
Molecule:mt-SAF15
Chain IDs:GA (auth: FG)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:mt-SAF16
Chain IDs:HA (auth: FH)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:mt-SAF17
Chain IDs:IA (auth: FI)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF18
Gene (Uniprot):TbgDal_XI11890
Chain IDs:D (auth: FJ)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:D0A8R9
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF19
Gene (Uniprot):Tb07.21H15.310
Chain IDs:JA (auth: FK)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57XS8 Pfam ID:PF02330
Polymer Type:polypeptide(L)
Molecule:mt-SAF20
Chain IDs:KA (auth: FL)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF25
Gene (Uniprot):Tb10.70.6030
Chain IDs:LA (auth: FV)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q38C60
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF28
Gene (Uniprot):DPX39_060023800
Chain IDs:E (auth: FY)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:A0A3L6L5M9
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF30
Gene (Uniprot):Tb07.29K4.100
Chain IDs:F (auth: Fa)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q57VU7
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:mt-SAF34
Gene (Uniprot):Tb11.03.0725
Chain IDs:MA (auth: Fe)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
UniProt ID:Q387L0
Polymer Type:polypeptide(L)
Molecule:UNK-a
Chain IDs:NA (auth: Ua)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-b
Chain IDs:OA (auth: Ub)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-c
Chain IDs:PA (auth: Uc)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-d
Chain IDs:QA (auth: Ud)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-e
Chain IDs:RA (auth: Ue)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-f
Chain IDs:SA (auth: Uf)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-g
Chain IDs:TA (auth: Ug)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-h
Chain IDs:UA (auth: Uh)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-i
Chain IDs:VA (auth: Ui)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-j
Chain IDs:WA (auth: Uj)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-k
Chain IDs:XA (auth: Uk)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-l
Chain IDs:YA (auth: Ul)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-m
Chain IDs:ZA (auth: Um)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Polymer Type:polypeptide(L)
Molecule:UNK-x
Chain IDs:AB (auth: Ux)
Chain Length:0
Number of Molecules:1
Biological Source:Trypanosoma brucei brucei
Ligand Molecules
GTP
MG
SAH
ZN
Primary Citation
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Science 365 1144 1149 (2019)
PMID: 31515389 DOI: 10.1126/science.aaw5570

Abstact

Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in Trypanosoma brucei involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.

Legend

Protein

Chemical

Disease

Primary Citation of related structures
6SG9
Feedback Form
Name
Email
Institute
Feedback